Composition having physical strength improving effect and/or anti-fatigue effect
A technology of composition and physical strength, applied in the direction of drug combination, application, microorganism, etc., can solve problems such as unexpectable effects
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Embodiment 1
[0076] Embodiment 1. the fishing bacteria of Bacteroidetes monomorpha
[0077] Human-derived feces were diluted, inoculated on a selective medium (NBGT agar medium), and cultured under anaerobic conditions for 48 hours.
[0078] Next, the obtained colonies were picked up one by one, suspended in PBS, a part of the suspension was inoculated on a BL agar medium, and cultured under anaerobic conditions for 48 hours. In addition, DNA was extracted from the remaining suspension, and quantitative PCR and 16S rRNA gene sequence determination were performed using the DNA as a template to identify the strain as Bacteroides monomorpha.
[0079] The identified strains were recovered from BL agar medium, inoculated on GAM soft agar medium, and cultured.
Embodiment 2
[0080] Embodiment 2. Preparation of Bacteroides monomorpha suspension
[0081] Two strains of Bacteroides monomorpha obtained in Example 1 were spread on BL agar medium and cultured for 48 hours. Next, for each strain, the obtained colonies were picked up one by one in a GAM liquid medium, and further cultured for 16 hours. The cultivation was carried out under anaerobic conditions. The obtained strains were referred to as "CP3585 strain" and "CP3586 strain", respectively.
[0082] After the cultivation, centrifugation was performed to remove the supernatant, and the recovered bacterial cells were frozen at -80°C and stored until administration.
[0083] When administering, the bacterium was mixed up to 1×10 9 Each bacterial cell suspension was prepared by diluting with PBS in the form of CFU / ml.
[0084] Furthermore, a suspension was prepared in the same manner using the JCM5828 strain which is a standard strain of Bacteroides monomorpha.
Embodiment 3
[0085] Example 3. Effects on one or more of physical strength and fatigue
[0086] (1) animals
[0087] Male C57BL / 6 mice (5 weeks old) were used.
[0088] Since there is a difference in the exercise ability of each individual, an extreme swimming test was performed once before the administration, and the mice were assigned to a control group, a JCM5828 strain-administered group, CP3585 strain administration group and CP3586 strain administration group (each n=8).
[0089] Each mouse was raised in a specific-pathogen free (SPF) environment.
[0090] (2) Administration of bacteria
[0091] During the experimental period (4 weeks), the suspension of any strain in the JCM5828 strain, CP3585 strain and CP3586 strain was treated with 2×10 strains every day. 8 The amount of CFU / day is mandatory for oral administration.
[0092] For the control group, the same amount of PBS was administered.
[0093] (3) Evaluation method
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