Pharmaceutical composition for treating laryngeal cancer as well as preparation method and application of pharmaceutical composition
A technology of composition and medicine, which is applied in the field of pharmaceutical composition for the treatment of laryngeal cancer, can solve the problems of few reports on pharmacological effects, and achieve good therapeutic effect
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Publication Date
- 2020-06-19
Smart Images
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Abstract
Description
technical field
[0001] The invention relates to the technical field of anticancer drugs, in particular to a pharmaceutical composition for treating laryngeal cancer, a preparation method and application thereof. Background technique
[0002] Traditional Chinese medicine is a precious cultural heritage of our country and has made great contributions to the cause of human health. my country has rich resources of Chinese herbal medicine and thousands of years of clinical experience in using natural medicines to prevent and treat diseases, but there is still room for exploration in the pharmacological effects or applications of some natural medicines. For example, Selaginella jiangnan, also known as "stone cypress", "rock cypress grass", "jaundice selaginella", etc., is derived from the whole herb of Selaginella moellendorfii Hieron, a plant of the family Selaginaceae. Plant form is a perennial evergreen herb with strong biological activity. Selaginella jiangnan is mainly dist...
Examples
Embodiment 1
[0081] The present embodiment provides a pharmaceutical composition, the active ingredients of which include the biflavones of Spiraea spiconiae, Ginkgo biflavones, Cypress biflavones, Bamboo biloba biflavones A, 7-desmethyl Ginkgo biflavones and 7,4',7",4 ”'-Tetramethoxy-Tetramethoxy-Taquinone, and the mass ratio is 52.7:7.8:4.7:2.3:8.2:2.5.
[0082] The embodiment of the present invention also provides a preparation method of the above-mentioned pharmaceutical composition, comprising:
[0083] refer to figure 1 The process flow shown is to extract Selaginella jiangnan to obtain a pharmaceutical composition containing the above active ingredients.
[0084] Specifically, the dried whole herb of Selaginella jiangnanensis (500 g) was ground, then immersed in 95% EtOH four times (2.5 L each time, 3 days) at room temperature for extraction. The solvent was evaporated under reduced pressure to obtain a crude extract (61.5 g). The crude extract was suspended in water by adding wa...
experiment example
[0088] The drug efficacy evaluation of the Selaginella chinensis biflavonoid enrichment provided in Example 1 of the present invention will be conducted below in combination with the drug efficacy test.
[0089] sample:
[0090] Sample to be tested: the Selaginella chinensis biflavone enrichment provided in Example 1;
experiment example 1
[0093] Experimental example 1, detection of the effect of the test article, comparative examples 1 and 2 on the viability of human laryngeal cancer cells Hep-2 and TU212 and normal laryngeal epithelial cells HBE cells, and the IC 50 value
[0094] Take Hep-2 and TU212 cell lines in the logarithmic growth phase, and adjust the cell concentration to 5×10 4 1 / mL, 0.1mL per well was inoculated into a 96-well culture plate, and after 24 hours of continuous culture, each well was added with 0.2mL of the sample to be tested prepared by DMEM solution containing 10% calf serum, so that the sample concentration was 5 μg / mL , 10 μg / mL, 20 μg / mL, 50 μg / mL and 100 μg / mL for 12 h, 24 h and 48 h; each concentration was replicated in 3 wells, and the experimental control without test samples and the blank control well without samples and cells were set up . Add 0.1mL MTT to each well and continue to incubate for 30min after the dosing time is reached. Discard the culture medium after the in...