Application of BAX PCR method in detection of salmonella in foods

Abstract

The invention relates to an application of a BAX PCR method in detection of salmonella in foods. The method comprises the following steps: step 1, putting a sample into bacterium enrichment broth to prepare an initial suspension; step 2, adding the bacterium enrichment broth extracted in the step 1 into the brain heart infusion broth, and carrying out secondary bacterium enrichment and re-culture;step 3, adding bacterium enrichment broth subjected to the secondary bacterium enrichment and the re-culture in the step 2 into a lysis tube, adding a protease-containing lysis solution into the lysis tube, heating the lysis tube in a heating tube, cooling the lysis tube in a cooling block, and taking and putting a lysis product into a PCR tube; and step 4, carrying out computer detection on thePCR tube in the step 3. The BAX PCR method provided by the invention is applied to detection of salmonella in foods, and rapidly carries out salmonella detection with a large batch of samples.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to the application of a BAX PCR method in the detection of food salmonella. Background technique [0002] Salmonella are a group of Gram-negative, facultative anaerobic bacteria that inhabit the intestinal tract of humans and animals. The antigen types of this genus are very complex. It is reported that there are more than 2,500 serotypes in the world, and more than 220 serotypes have appeared in my country. As a common food-borne pathogen, it is very common to contaminate most animal-derived foods such as eggs, livestock and poultry meat, and fresh milk. It also exists widely in the natural environment and is also a cause of food-borne bacterial diseases. primary pathogenic bacteria. There are hundreds of thousands of people infected with Salmonella every year in Europe, and more than one million people infected with Salmonella every year in the United States. In my countr...

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Problems solved by technology

[0003] The traditional Salmonella detection method mainly uses selective medium and biochemical reaction characteristics to detect, which has a long cycle, cumbersome process, and low sensitivity, and it is difficult to meet the rapid needs of food hygiene inspection.

There are following defects: 1) The traditional method requires at least 6 kinds of isolation culture media, nearly 10 kinds of identification reagents and expensive identification strips; 2) The complexity of Salmonella antigen types and the diversity of biochemical characteristics make it difficult to judge the identification results. 3) There are many kinds of food substrates, and the influence of substrate inhibition emerges endlessly, and the sensitivity of traditional methods is difficult to meet; 4) The new selective chromogenic medium contains High-strength antibacterial substances, long-term exposure will cause certain harm to the human body; 5) Food safety has been paid more and more attention, and testing requirements have been continuously strengthened. The demand for large-scale samples and large-volume sampling volumes is increasing, and traditional methods are difficult to efficiently meet

[0004] In recent years, researchers have devoted themselves to the development of new technologies for the detection of Salmonella, and have made great progress from the time-consuming and laborious traditional isolation and culture methods to immunological detection methods, and then to molecular biology detection techniques, and have achieved remarkable results. This application The purpose of this paper is to provide an application of BAX PCR method with high detection efficiency in the detection of Salmonella in food

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