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Rapid rooting and seedling-forming method for psammosilene tunicoides by water culture

A hydroponic rooting and water-locking technology, applied in botany equipment and methods, cultivation, soilless cultivation, etc., can solve the problems of reducing the quality and emergence rate of Jintiesuo seeds, uncertain commercial life of Jintiesuo rhizomes, inconsistent seed maturity, etc. , to achieve the effect of reducing the cost of rooting culture, low cost and high cost

Active Publication Date: 2020-06-23
云南省农业科学院高山经济植物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the seed propagation of Jintiesuo mainly has the following problems: First, because the inflorescence of Jintiesuo is an infinite inflorescence, there are problems of different seed maturity periods, so that the maturity of each seed is inconsistent, and most people fail to do it during the harvesting of seeds. Harvested in stages, some of the seeds were shriveled, which reduced the quality and emergence rate of Jin Tiesuo seeds as a whole; second, the emergence of the seeds after sowing was poor, and the emergence period was as long as one month, and the emergence was irregular
The third is that Jintiesuo is a perennial plant, and the seeds are easy to fall off after maturity. Every year, seeds fall in the field to germinate naturally, resulting in an uncertain commodity life of Jintiesuo roots in the field.
There are also relatively many reports on the method of rooting and seedling growth through tissue culture of Jin Tiesuo, but there are also defects such as long cycle time and high cost.

Method used

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  • Rapid rooting and seedling-forming method for psammosilene tunicoides by water culture
  • Rapid rooting and seedling-forming method for psammosilene tunicoides by water culture
  • Rapid rooting and seedling-forming method for psammosilene tunicoides by water culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Select stems of different periods as test materials: ① After harvesting all the seeds of the annual Jin Tiesuo in the seed harvesting period, take its 5-8 cm green leafy stem (abbreviation: annual stem); ② Biennial After all the seeds of Jintiesuo are harvested during the seed harvesting period, take the 5-8cm green leafy stem section; stem).

[0022] (2) Stem soaking time for 50% carbendazim wettable powder 1000 times liquid disinfection: ① 0.5 hours; ② 1 hour; ③ 1.5 hours; ④ 2 hours.

[0023] The experimental results show:

[0024] (1) "Annual stems" and "biennial young tender stems" are soaked in 50% carbendazim wettable powder 1000 times solution for 1 to 2 hours. After the seeds are harvested, there is no such phenomenon in taking the 5-8cm green leafy stem section. Therefore, it is advisable to select the green leafy stem section of the two-year-old Jintiesuo after all the seeds are harvested during the seed harvesting period as Jintiesuo hydroponic rooting ...

Embodiment 2

[0027] 1. Materials and methods

[0028] (1) Select the two-year-old Jin Tiesuo in its seed harvesting period. After all the seeds have been harvested, take the green leafy stems with a stem length of more than 10cm, and then cut them into: ①The stem segments with a length of 5-8cm, each stem segment Keep 3 pairs of buds and 3 pairs of leaves; ② For 5-8cm long stem segments, keep 3 pairs of buds for each stem segment without leaves; ③ For 2-2.5cm long stem segments, keep 1 pair of buds for each stem segment bud points and 1 pair of leaves; ④ stem segments with a length of 2 to 2.5 cm, each stem segment retains 1 pair of bud points without leaves. 120 stem segments of each type.

[0029] (2) Put the cut stem sections of various specifications into 50% carbendazim wettable powder 1000 times liquid respectively and soak them for 1 hour for disinfection (the whole stem section including the leaves and buds on the stem sections are all submerged in the described carbendazim each ...

Embodiment 3

[0044] Compare the method of the present invention with the conventional seed seedling raising method and group seedling raising method of Jin Tiesuo:

[0045] 1. Materials and methods

[0046] (1) By adopting the method of the present invention, put the stem segments (120 pieces) with a length of 5 to 8 cm, retaining 3 pairs of bud points and 3 pairs of leaves into 50% carbendazim wettable powder 1000 times liquid and soak for 1 hour after disinfection , insert in the culture tank that is placed with hydroponic rooting culture solution (IBA solution of 1ppm), the length of the incision under the stem section soaked in the culture solution is 0.5cm; finally put into the artificial climate box: ① temperature 22 ℃, humidity 60% , light intensity 15000lx, 16h; ②Temperature 16°C, humidity 60%, light intensity 0lx, rooting culture was carried out in 8h; rooting situation was observed regularly and the rooting culture medium was replaced every 7 days. Transplant the seedlings with ...

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Abstract

The invention discloses a rapid rooting and seedling-forming method for psammosilene tunicoides by water culture. The method comprises the following steps: selecting biennial psammosilene tunicoides,after all seeds of the psammosilene tunicoides are harvested in a seed harvesting period, taking green stem sections with leaves and a length of 5-8 cm from the psammosilene tunicoides, reserving three pairs of bud points and three pairs of leaves in each stem section, carrying out disinfection treatment with carbendazim, and carrying out culturing in a water-culture rooting culture solution underthe condition of an artificial climate box, wherein the water-culture rooting culture solution is an IBA solution with a concentration of 0.5-1 ppm, and the culture conditions set by the artificial climate box are as follows: (1) a temperature of 22 DEG C, a humidity of 60%, an illumination intensity of 15,000 lx and the culture time of 16 hours; and (2), a temperature of 16 DEG C, a humidity of60%, an illumination intensity of 0 lx and the culture time of 8 hours. The method provided by the invention has the advantages of rapid rooting, large root system amount, strong root system, high seedling forming rate and low cost, solves the problems of poor seedling emergence potential, long seedling emergence period, low seedling forming rate and the like for seedling raising of psammosilene tunicoides seeds, and provides a technical support for industrial rapid propagation of the psammosilene tunicoides in the future.

Description

technical field [0001] The invention belongs to the technical field of medicinal plant breeding, and in particular relates to a method for rapid rooting and seedling formation of Jin Tiesuo in hydroponics. Background technique [0002] Psammosilene tunicoides (Psammosilene tunicoides) is a single-species perennial herb of Caryophyllaceae. Jintiesuo has important medicinal value. Its dried roots are used as medicine in the folks. It has the functions of dispelling blood stasis, relieving pain, dispelling rheumatism, and reducing inflammation. , Jingulian Capsules, Baibaodan, Tongxuekang Capsules and other raw materials of Chinese patent medicines. The distribution area of ​​wild resources of Jintiesuo is narrow and the quantity is small, coupled with a large number of human excavation, the distribution of wild resources has decreased sharply, and it has been listed as a national second-class protected plant. In order to meet the demand of Jintiesuo medicinal materials marke...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00
CPCA01G31/00
Inventor 和桂青陈翠戚淑威和根强程远辉杨少华杨丽云和琼姬康平德
Owner 云南省农业科学院高山经济植物研究所
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