Microfluidic chip optical positioning code disc, device and method

Abstract

The invention discloses a microfluidic chip optical positioning code disc, device and method. The cross section of the outer contour of the code disc is circular, N light-transmitting openings are uniformly formed in the periphery of the code disc, and the length of the first light-transmitting opening is larger than that of the second to N light-transmitting openings. The device comprises the code disc, a positioning pin, a rotating shaft, a motor, an internal photoelectric switch and an external photoelectric switch, wherein a positioning surface is arranged on the rotating shaft; the motoris fixedly connected with one end of the rotating shaft; the other end of the rotating shaft penetrates through the center of the cross section of the code disc, and when the code disc is used, the positioning pin is arranged at a positioning notch of a microfluidic chip to be matched with the positioning surface for positioning; and the internal photoelectric switch and the external photoelectricswitch are used for identifying the light-transmitting openings in the code disc. According to the code disc, the microfluidic chip is positioned by adopting optical identification, so that the requirement for the stability of the operation rotating speed of the motor is reduced, and the measurement data is more accurate and reliable.

Description

technical field [0001] The invention relates to the technical field of microfluidic chip positioning, in particular to a microfluidic chip optical positioning code disc, device and method. Background technique [0002] Nucleic acid analysis is widely used in life sciences, medicine, food safety, environmental monitoring and other fields. This method uses a microfluidic chip detection technology, which integrates discontinuous processes such as sample preparation, biochemical reaction, and analysis and detection in a microfluidic chip, and realizes the overall miniaturization, automation, and integration from sample reaction to result detection. and portability. How to correctly and effectively collect fluorescence signal data during nucleic acid amplification has become a key factor in nucleic acid analysis. [0003] Such as figure 1 As shown, the microfluidic chip is composed of N (N=24 in a common microfluidic chip) reaction pools, each reaction pool has a corresponding...

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Problems solved by technology

[0004] This method has high requirements on the stability of the stepping motor speed, because the change of the motor speed will affect the data distribution and the offset of the required measurement points, which will affect the accuracy of the fluorescence data

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