Variant p53 gene and recombinant adenovirus for cancer treatment

Abstract

The invention discloses a variant p53 gene for cancer treatment. A cDNA base sequence of the p53 gene is constructed by replacing codon 72 in a cDNA base sequence of a naturally occurring p53 gene with a target codon. The invention also discloses a recombinant adenovirus containing the variant p53 gene. The recombinant adenovirus is constructed by artificially synthesizing a DNA target fragment containing the target codon, replacing the DNA target fragment with a DNA fragment containing a p53 expression cassette in an intermediate plasmid to generate a variant intermediate plasmid containing the variant p53 gene, and introducing the DNA fragment containing the variant p53 gene in the variant intermediate plasmid into a shuttle plasmid, and then performing homologous recombination to obtainthe recombinant adenovirus containing the variant p53 gene.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and relates to a series of variant p53 genes used for gene enhancement, addition and complementary cancer treatment, and a construction method of a recombinant adenovirus containing the variant p53 gene. Background technique [0002] The use of genes to treat various human diseases has been envisioned since it was established that defective gene function is a major cause of genetic diseases. In most cases, the cause of a gene defect is likely to be due to a mutation that affects its protein structure and forces it not to function properly, or a mutation that affects the expression level of the protein leading to a complete failure of the protein to highlight or improper expression during the cell cycle . Therefore, the goal of gene therapy is to introduce therapeutic genes into target cells and repair those disease-inducing genes, so as to restore the normal function of target cells ...

AI Technical Summary

Problems solved by technology

In most cases, the cause of a gene defect is likely to be due to a mutation that affects its protein structure and forces it not to function properly, or a mutation that affects the expression level of the protein leading to a complete failure of the protein to highlight or improper expression during the cell cycle

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