Construction method and quality inspection method of ULC characteristic map of wind-resistant medicinal materials

A construction method and a technology of characteristic maps, applied in the field of construction of UPLC characteristic maps, can solve the problems of not being able to comprehensively evaluate the quality of windproof and not fully reflect the material characteristics of traditional Chinese medicine decoctions, and achieve the effect of overcoming the deviation of durability

Active Publication Date: 2022-08-05
SINOPHARM GUANGDONG GLOBAL PHARMA CO LTD
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0004] "Chinese Pharmacopoeia" 2015 edition of Fangfeng medicinal materials contains content determination items of acteoside and 5-O-methylvisamigoside, etc., which cannot comprehensively evaluate the quality of Fangfeng
At present, most of the fingerprints or characteristic maps of Fangfeng reported in the literature are conventional HPLC methods, and they only focus on the material basis of the original medicinal materials. Most of the index components are fat-soluble components as the research objects, which cannot fully reflect the substances of traditional Chinese medicine decoctions. basic features

Method used

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Experimental program
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Effect test

Embodiment 1

[0047] Embodiment 1, the construction method of the UPLC characteristic map of windproof medicinal material

[0048] The present embodiment provides a method for constructing a UPLC characteristic map of windproof medicinal materials, including the following:

[0049] 1. Instruments and reagents

[0050] Table 1. Instrument information table

[0051]

[0052]

[0053] Table 2. Reagent information table

[0054] Reagent name factory level batch number Chromatography Acetonitrile Shanghai Xingke HPLC grade 011470303 Chromatography methanol BCR HPLC grade 170320104001 water M MILLIPORE Synergy UV Ultra-pure water the day

[0055] Table 3. Reference substance information table

[0056]

[0057] Table 4. Information table of windproof medicinal materials

[0058]

[0059]

[0060] In the table, Cimicifolin glycosides and 5-O-methyl visamidol glycosides were determined according to the content determination meth...

Embodiment 2

[0191] Embodiment 2, a kind of quality detection method of windproof medicinal material

[0192] The present embodiment provides a quality detection method for windproof medicinal materials, comprising the following steps:

[0193] 1. The preparation of the solution and the chromatographic conditions are the same as those in "2. Chromatographic conditions and preparation of the test solution" in "Example 1".

[0194] 2. Determination Precision Absorb 1 μl of the test solution, inject it into the liquid chromatograph, and measure it.

[0195] Compare the characteristic spectrum of the medicinal herbs to be tested with the control characteristic spectrum of the medicinal herbs: the sample to be tested can detect 5 characteristic peaks, which should correspond to the 5 characteristic peaks in the chromatogram of the reference substance of the control medicinal material, and the The peak corresponding to the reference peak is the S peak. Calculate the relative retention time of...

Embodiment 3

[0196] Embodiment 3, the identification of wild windproof and cultivated windproof medicinal materials

[0197] The present embodiment provides a method for identifying wild windbreaks and cultivated windbreaks, including the following:

[0198] 1. Test drug

[0199] Table 23. List of medicinal materials for windproofing

[0200] serial number Medicinal batch number Remark 1 YSFF01 wild 2 YSFF02 wild 2 YSFF03 wild 3 YSFF04 wild

[0201] 2. The preparation of the solution and the chromatographic conditions are the same as those in "2. Chromatographic conditions and preparation of the test solution" in "Example 1".

[0202] 3. Determination Precision Absorb 1 μl of the test solution, inject it into the liquid chromatograph, and measure it.

[0203] 4. Results

[0204] 5 characteristic peaks can be detected in 4 batches of wild parsnip samples, which correspond to the 5 characteristic peaks in the chromatogram of the referen...

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Abstract

The invention relates to a construction method and a quality detection method of a UPLC characteristic map of windproof medicinal materials. The construction method includes: reference substance solution preparation: preparing a reference substance solution of a reference medicinal material; preparing a reference substance solution of cichoside and 5-O-methyl visamidol glycosides; preparation of a test solution: taking Fangfeng Medicinal materials, solvent extraction, filtration, and the obtained filtrate was used as the medicinal material test solution; Fangfeng standard decoction was taken, solvent extraction, and filtration, and the obtained filtrate was used as the decoction test solution; Ultra-high performance liquid chromatography detection: Absorb reference medicinal materials for reference The substance solution, reference substance reference substance solution, medicinal material test solution, decoction test solution were injected into a liquid chromatograph, and the obtained medicinal material test sample spectrum and decoction test sample spectrum were compared, and the UPLC characteristic spectrum was obtained. The characteristic map can be used for qualitative and quantitative analysis of the quality of the Fangfeng medicinal material, can ensure the quality of the traditional Fangfeng decoction prepared by using the medicinal material, and is also suitable for detecting the decoction.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for constructing a UPLC characteristic map and a method for quality detection of windproof medicinal materials. Background technique [0002] Fangfeng is the dried root of Saposhnikoviadivaricata (Turcz.) Schischk, a Umbelliferae plant, and is commonly used in clinical Chinese medicine. It has the functions of expelling wind and relieving external appearance, eliminating dampness and relieving pain, and relieving spasm. It is mostly used clinically to treat colds and headaches, rheumatic arthralgia, itching of rubella, tetanus, etc. It has strong clinical application value and broad market demand. Relevant compound preparations such as Yupingfeng Granules and traditional Chinese medicine formula granules are widely used in clinical practice. [0003] The clinical use of traditional Chinese medicine is mainly based on traditional decoction. The material basis of tradit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/047
Inventor 张文芳林碧珊陈锦霞史紫娟曾杉陈伟钢甘伟发高永坚袁春平汤春花
Owner SINOPHARM GUANGDONG GLOBAL PHARMA CO LTD
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