Nucleic acid dye, and preparation method and application thereof

A nucleic acid dye and nucleic acid technology, applied in the field of gel imaging staining and new nucleic acid dyes, to achieve the effect of maintaining sensitivity, accuracy and sensitivity

Active Publication Date: 2020-07-03
UELANDY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention discloses a nucleic acid dye and its preparation method and application; as a nucleic acid dye, compared with existing products, the compound of the invention not only maintains a unique high sensitivity, but also is safer and non-toxic, especially solving the problem of existing nucleic acid dyes. Tailing phenomenon in electrophoresis test, especially can be applied to agarose gel electrophoresis and polyacrylamide gel electrophoresis at the same time, especially blue light imaging

Method used

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  • Nucleic acid dye, and preparation method and application thereof
  • Nucleic acid dye, and preparation method and application thereof
  • Nucleic acid dye, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Preparation of nucleic acid dye

[0051] 1. Add 25 g of 2-(methylthio)benzothiazole and 30.8 g of methyl p-toluenesulfonate to a 500 mL reaction flask, stir mechanically, heat to 60°C, and track by TLC until the end of the reaction (developing solvent: acetonitrile: Water=5:1); After the reaction, add 300 mL of ethyl acetate, heat up to 75°C and reflux for 3 hours; then cool down to room temperature, filter, wash the filter cake with ethyl acetate once more, filter, and vacuum dry the filter cake to obtain the product 47 g Intermediate I.

[0052]

[0053] in:

[0054] 2-(methylthio)benzothiazole:

[0055]

[0056] Methyl p-toluenesulfonate:

[0057]

[0058] Intermediate I:

[0059]

[0060] 2. Add 11.76 g of 4-methylquinoline, 28 g of bromo-triethylene glycol-carboxylic acid, and 10 mL of o-dichlorobenzene into a 250 mL reaction flask, heat to 120°C, and follow TLC until the end of the reaction ( The developer is: acetonitrile: water = 5:2);...

Embodiment 2

[0077] Example 2 By replacing the raw materials, other nucleic acid dyes in Table 1 can be prepared, specifically, conventional replacements.

[0078] For example, replace the ethylenediamine in the step (4) of Example 1 with the compound of formula ① or the compound of formula ②, or the compound of formula ③, and keep the rest unchanged, to obtain the compounds of No. 2, No. 3 and No. 4 in Table 1, wherein The chemical formulas of the compound of formula ①, the compound of formula ②, and the compound of formula ③ are as follows respectively: H 2 N(CH 2 CH 2 O) 3 CH 2 CH 2 NH 2 、H 2 NCH 2 CH 2 OCH 2 CH 2 NH 2 、H 2 NOCH 2 CH 2 ONH 2 .

[0079] For example, replace the ethylenediamine in the step (4) of Example 1 with the compound of formula 1 or the compound of formula 2, and keep the rest unchanged, to obtain the compound of No. 7 and No. 8 in Table 1, wherein the compound of formula 1 and the compound of formula 2 The structural formulas of the compounds are ...

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Abstract

The invention discloses a nucleic acid dye, and a preparation method and an application thereof. The nucleic acid dye is used for common gel imaging dyeing of nucleic acid electrophoresis, and concretely is a novel nucleic acid dye which can be simultaneously suitable for agarose gel electrophoresis and polyacrylamide gel electrophoresis, and particularly can be used for blue light imaging. The invention also relates to the preparation and the application of dye. The developed novel nucleic acid dye is good in dyeing effect, high in safety, very low in nucleic acid migration influence rate andwide in applicability and can realize blue light imaging. The defect that an existing nucleic acid dye cannot be applied to polyacrylamide gel electrophoresis due to a poor dyeing effect is effectively overcome, and particularly the problem that tailing is likely to be generated when the existing dye is used for hair dyeing and electrophoresis is solved.

Description

technical field [0001] The invention is used for common gel imaging staining of nucleic acid electrophoresis, specifically a novel nucleic acid dye that can be applied to both agarose gel electrophoresis and polyacrylamide gel electrophoresis, especially for blue light imaging, and relates to the preparation and application of dyes . Background technique [0002] Nucleic acid electrophoresis is an important means of nucleic acid research and an indispensable part of nucleic acid probes, nucleic acid amplification and sequence analysis technologies. Nucleic acid electrophoresis usually uses two media, agarose gel and polyacrylamide gel. Agarose is a chain-like polysaccharide prepared by separation from agar. Many agaroses are coiled with each other to form rope-like agarose bundles according to the action of hydrogen bonds and other forces, forming a large mesh gel, suitable for separation and purification of 200bp-50kB in length It can distinguish DNA fragments with a diff...

Claims

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Application Information

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IPC IPC(8): C09B23/04C07D417/14G01N27/447G01N1/30C12Q1/68
CPCC07D417/14C09B23/04C12Q1/68G01N1/30G01N27/447G01N27/44747G01N2001/302C12Q2565/125
Inventor 周杰郑慧敏马晓峰谈雪良钱近春夏继波
Owner UELANDY INC
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