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Automated assay device configured to disrupt cell in sample

A technology for measuring devices and samples, which is applied in the preparation of test samples, measuring devices, and determination/inspection of microorganisms, and can solve the problems of time-consuming, difficult to implement, and high cost.

Inactive Publication Date: 2020-07-03
THERANOS IP CO LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, existing methods for detecting or identifying pathogens are often inaccurate, difficult to implement, costly and time consuming

Method used

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  • Automated assay device configured to disrupt cell in sample
  • Automated assay device configured to disrupt cell in sample
  • Automated assay device configured to disrupt cell in sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0178] Example 1 System for Disrupting Pathogens Including Ultrasonic Apparatus

[0179] Embodiments of the systems disclosed herein include a device having a sonicator configured to apply ultrasonic energy to a sample solution. Application of ultrasonic energy to a sample solution containing a pathogen is effective to disrupt the pathogen and release the pathogen-identifying substance into the solution where it can be detected and the pathogen identified. In embodiments, the amount of pathogen present in the sample solution may be quantified after application of ultrasonic energy to effectively disrupt the pathogen and release the pathogen-identifying substance into the solution. figure 1 A schematic diagram representing elements of an embodiment of a system comprising a device with an ultrasound machine is shown in .

[0180] In an embodiment, the tip portion of the sonicator of the device of the system as disclosed herein is configured to improve the delivery of ultrasoni...

Embodiment 2

[0188] Embodiment 2 ultrasonic instrument and utensil

[0189] exist figure 2 and image 3 An example of an ultrasound machine suitable for use in the present devices, systems and methods is shown in . figure 2 A diagram of an embodiment may be provided in which the distal portion of the ultrasound machine 40 (e.g., the tip 44 of the ultrasound machine horn 42 shown within the vessel 46) - referred to in this example as "tip" 44 - - contacting the sample solution in the vessel 46 . In such embodiments, tip 44 contacts the sample solution. Direct contact with the sample solution provides direct energy transfer from the sonicator 40 to the sample solution. However, such direct contact requires the tip 44 to touch or enter the sample solution, thus providing a possible source of contamination of the sample (and subsequent sample), and requires a cleaning step and / or coating of the tip 44 to reduce such contamination. pollute.

[0190] figure 2 An illustration of an embo...

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PUM

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Abstract

Devices, systems and methods including a sonicator for sample preparation are provided. A sonicator may be used to mix, resuspend, aerosolize, disperse, disintegrate, or de-gas a solution. A sonicatormay be used to disrupt a cell, such as a pathogen cell in a sample. Sample preparation may include exposing pathogen-identifying material by sonication to detect, identify, or measure pathogens. A sonicator may transfer ultrasonic energy to the sample solution by contacting its tip to an exterior wall of a vessel containing the sample. Multipurpose devices including a sonicator also include further components for additional actions and assays. Devices, and systems comprising such devices, may communicate with a laboratory or other devices in a system for sample assay and analysis. Methods utilizing such devices and systems are provided. The improved sample preparation devices, systems and methods are useful for analyzing samples, e.g. for diagnosing patients suffering from infection by pathogens.

Description

[0001] This application is an invention with an international filing date of March 10, 2014, an international application number of PCT / US2014 / 022847, an application number of 201480027228.8 entering the Chinese national phase, and an invention titled "Apparatus, system and method for sample preparation" A divisional application of a patent application. Background technique [0002] Rapid and accurate detection and identification of pathogens such as bacteria is important and may be critical in the diagnosis and treatment of infectious diseases and in the prevention or mitigation of pandemics and epidemics. However, methods for detecting or identifying pathogens often require large samples, which may be difficult to obtain, or may cause discomfort or pain to the subject. Methods for the detection or identification of pathogens often require incubation of samples in growth media, or in host cells or in host animals, and therefore take a long time. Many pathogens are difficult ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N21/01G01N21/03
CPCG01N1/286G01N1/38G01N35/00871G01N35/1009G01N2001/4094G01N2035/00554G01N33/487G01N2035/00524B01L3/5082B01L2200/025B01L2300/0858B01F31/86C12Q1/04G01N1/28G01N35/00
Inventor A·罗E·A·霍姆斯
Owner THERANOS IP CO LLC