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Non-coding RNA-LOC105376991 for diagnosing osteoporosis

An osteoporosis, long-chain non-coding technology, applied in the field of biomedicine, can solve the problems of low sensitivity and accuracy of osteoporosis, and little help for early diagnosis of osteoporosis

Inactive Publication Date: 2020-07-07
GUAN BOJIAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, plain X-ray films have low sensitivity and accuracy in diagnosing osteoporosis, and are of little help in the early diagnosis of osteoporosis.

Method used

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  • Non-coding RNA-LOC105376991 for diagnosing osteoporosis
  • Non-coding RNA-LOC105376991 for diagnosing osteoporosis
  • Non-coding RNA-LOC105376991 for diagnosing osteoporosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 Screening for differentially expressed molecules

[0065] 1. Case collection

[0066] Five elderly male patients with osteoporosis were selected, aged 62-79 years; the diagnosis of osteoporosis was based on the standards established by the Osteoporosis Committee of the Chinese Society of Geriatrics, that is, the bone mineral density at the same site was lower than the peak value of the area and decreased by two standard deviations classified as osteoporosis. During the same period, 6 healthy elderly men (healthy control group) were selected, aged 61-78 years. The selected objects exclude diabetes, primary hyperparathyroidism, hyperthyroidism, hypercortisolism, liver and kidney dysfunction, alcoholism, malnutrition, rheumatoid arthritis and other diseases that affect bone metabolism; no application within half a year Androgens, calcitonin, large amounts of calcium, bisphosphonates, and other drugs that affect bone metabolism. The two groups were comparable i...

Embodiment 2

[0107] Example 2 Large sample verification screened out differentially expressed LncRNA

[0108] 1. Sample collection

[0109] According to the method of Example 1, 32 cases of osteoporosis patients and 30 cases of male healthy controls were collected.

[0110] 2. Extract blood total RNA

[0111] Step is with embodiment 1.

[0112] 3. Validation at the RNA level

[0113] (1) Reverse transcription: According to the measured RNA concentration, 1000 ng of RNA was quantified for each sample for reverse transcription. Use the DDR037A reverse transcription kit of Dalian Biobiology, add 20 μl of the system (see Table 1), add it to a 200ul enzyme-free EP tube, centrifuge and put it into a PCR instrument, and set the temperature conditions as follows: 37°C for 15min, 85°C for 5sec . After the end, the cDNA was diluted 10 times and stored at -20°C for future use.

[0114] Table 1 Reverse transcription system

[0115]

[0116] (2) Real-time quantitative PCR

[0117] Shanghai S...

Embodiment 3

[0130] Example 3 Effect of LOC105376991 Expression on Osteoblast Proliferation and Differentiation

[0131] 1. Overexpression pre-experiment

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Abstract

The invention discloses a non-coding RNA-LOC105376991 for diagnosing osteoporosis. In addition, the invention further discloses a product for diagnosing the osteoporosis. The product achieves the target of diagnosis through detecting LOC105376991 in blood. A study proves that the level of the LOC105376991 in the blood of a patient with the osteoporosis was significantly reduced in comparison withthat in a normal person. According to the correlation between the LOC105376991 and the osteoporosis, a kit for diagnosing the osteoporosis can be prepared, and the kit can be widely used in clinic.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a non-coding RNA for diagnosing osteoporosis, and the non-coding RNA is LOC105376991. Background technique [0002] The incidence of osteoporosis in men is much lower than that of postmenopausal women, so in the past, less attention was paid to men's osteoporosis than to women. Research results in recent years have shown that although young men can achieve a higher peak bone mass than women, and the start time of bone loss is also significantly later than women, but both men and women have a bone loss that is phased with age Process, that is, senile osteoporosis (type II). There are many similarities between male osteoporosis and female osteoporosis, but there are still obvious gender differences in etiology, pathology, and disease. Osteoporosis of varying degrees is common in men over 65 years old, and its serious complication is fracture. [0003] In the United States, the incidence ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6837C12N15/113C40B40/06
CPCC12Q1/6837C12Q1/6883C12Q2600/178C40B40/06
Inventor 杨承刚向常娟
Owner GUAN BOJIAN BIOTECH CO LTD
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