Tissue culture and bulb regeneration method of Xinjiang wild tulip

A technology for wild tulips and tulips, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of slow technological development and achieve the effects of improved reproductive efficiency, low pollution rate, and high reproductive coefficient

Active Publication Date: 2020-07-10
HUAZHONG AGRI UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are not many reports on tulip tissue culture and genetic transformation...

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  • Tissue culture and bulb regeneration method of Xinjiang wild tulip
  • Tissue culture and bulb regeneration method of Xinjiang wild tulip
  • Tissue culture and bulb regeneration method of Xinjiang wild tulip

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Embodiment Construction

[0022] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0023] The method for tissue culture and bulb regeneration of Xinjiang wild tulip comprises the following steps:

[0024] (1) Harvest mature Xinjiang wild tulip capsules in mid-to-late May and dry them in the sun.

[0025] (2) Peel off the air-dried Ili tulip capsules, take out the flaky and brown seeds inside, and place them on a clean white paper. Remove shriveled seeds as well as white immature seeds.

[0026] (3) Rinse the flaky brown seeds in running water for 1-2 hours, disinfect with 75% alcohol for 1 minute in an ultra-clean workbench, rinse with sterile water twice, then disinfect with 2% sodium hypochlorite for 20 minutes, rinse with sterile water 6-8 times until the water becomes clear.

[0027] (4) Pl...

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Abstract

The invention relates to a tissue culture and bulb regeneration method of Xinjiang wild tulip. The method comprises the following steps: 1, selecting tulip seeds, taking out a seed embryo, dividing the seed embryo into two halves, inoculating the two halves into an induction culture medium for dark culture, and transferring the two halves into a light condition for callus induction and proliferation; 2, inoculating the callus into a first differentiation culture medium for light culture, and growing regeneration buds on the callus; 3, subculturing the callus with bud points on a second differentiation culture medium to carry out illumination culture to obtain tissue culture seedlings; and subculturing the callus with bud points on a third differentiation medium for low-temperature dark culture to obtain regenerated bulblets. According to the method, the Xinjiang wild tulip seed embryo is taken as an explant, the explant material is rich, the pollution rate is low, the regeneration capability of the induced callus is strong, tissue culture seedlings and regenerated bulblets are obtained through callus induction, the propagation coefficient is high, limitation of seasons is avoided,the tulip bulb propagation efficiency can be greatly improved, and the growth period is shortened.

Description

technical field [0001] The invention relates to the field of rapid plant propagation, in particular to a method for tissue culture and bulb regeneration of Xinjiang wild tulips. Background technique [0002] Tulip is a world-famous bulbous flower. It is native to Xinjiang, the Mediterranean coast, Central Asia and Turkey. It is currently planted all over the world, and the breeding and cultivation techniques in the Netherlands are the most mature. Xinjiang is one of the distribution areas of tulips in the world. There are 12 species of wild tulips, mainly distributed in the Junggar Basin in northern Xinjiang, such as Ili tulip (T.iliensis) and Junggar tulip (T.buhseana). Yili tulips are mostly born in the deserts and steppes of the piedmont plains, and are often distributed in large areas. They are the dominant species of early spring vegetation. This species has a wide distribution and different habitats, so the plant height, leaf shape and flower size vary greatly with di...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 产祝龙王艳平孙琪
Owner HUAZHONG AGRI UNIV
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