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Gynogenetic black carp culture method

A breeding method and gynogenesis technology, applied in the field of fish breeding, can solve the problems of difficult gynogenesis, long breeding cycle, lack of good varieties with disease resistance ability, etc., to simplify the detection difficulty, have excellent characters, and improve the hatching rate. and offspring survival

Active Publication Date: 2020-07-10
HUNAN NORMAL UNIVERSITY
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Problems solved by technology

Herring is the first of the "four major family fishes" in my country. Due to its long breeding cycle (5-7 years for female sexual maturity and 4-6 years for male sexual maturity), it is difficult to develop gynogenesis (herring has not yet developed gynogenesis). Successful reports), there is still a lack of fine varieties with strong disease resistance across the country, so the development of innovative breeding techniques is of great significance for the selection and breeding of new herring varieties

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  • Gynogenetic black carp culture method
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  • Gynogenetic black carp culture method

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Embodiment

[0033] A method for cultivating gynogenetic herring of the present invention, comprising the steps of:

[0034]1. Artificial induced labor: From January to February every year, select herrings over six years old, without sickness, well-developed, and obvious sexually mature characteristics, and male koi over two years old, without sickness, well-developed, and obvious sexually mature characteristics, and put them into Artificial intensive breeding in broodstock ponds, the proportion of natural baits such as snails and clams fed daily to herring ponds should not be lower than 70% of the total bait; in mid-to-late May, when the pond water temperature stabilizes between 22 and 28°C 10-15 days before induction, each herring was injected with a luteinizing hormone-releasing hormone analogue (LRH-A 2 ) to induce ripening; during labor induction, the female herring is injected twice, and the first injection is to inject LRH-A at a dose of 5 μg / kg per fish 2 10-12h later, each tail w...

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Abstract

The present invention discloses a gynogenetic black carp culture method. The method comprises the following steps of firstly selecting sexually mature male fancy carp and female black carp for artificial spawning, then mixing ultraviolet inactivated sperms of the fancy carp with mature ova of the black carp, activating the ova, performing cold shock treatment on the activated ova at 0-4 DEG C for12-15 minutes, performing treatment in water with the temperature of 8-10 DEG C and water with the temperature of 16-18 DEG C for 1-2 minutes, performing incubation in an incubation tank with the water temperature of 25-28 DEG C, transferring incubated fish fries into a pond for culture, and performing identification and screening to obtain the diploid gynogenetic black carp. The method effectively overcomes the technical obstacle that viable gynogenetic black carp is difficultly obtained by a conventional method, gynogenetic black carp schools are successfully obtained, and the blank of a black carp gynogenesis technology is filled.

Description

technical field [0001] The invention belongs to the technical field of fish cultivation, in particular to a method for cultivating gynogenetic herring. Background technique [0002] Gynogenesis is an important genetic breeding method and an effective way to quickly establish homozygous lines. One to two generations of gynogenesis is equivalent to 8 to 10 generations of continuous breeding of traditional inbreeding. Through gene purification, individuals carrying harmful genes are naturally eliminated during gynogenesis, so the offspring of gynogenesis often show excellent traits such as disease resistance, strong stress resistance, and fast growth. [0003] Green carp, grass carp, silver carp, and bighead carp are the traditional "four major carp" in China. They have made important contributions to my country's food security for a long time. Their breeding scale is large and the output is high. In 2017, the total output of the "four major carp" reached 12.981 million tons , ...

Claims

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Application Information

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IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 冯浩肖军肖俊吴慧
Owner HUNAN NORMAL UNIVERSITY
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