Method for extracting flavone from holboellia latifolia
A flavonoid and powder technology, which is applied in the field of plant active ingredient extraction, can solve the problems of peculiar smell of gum, high energy consumption, limited application, etc., and achieves the effects of increasing total flavonoid content, good water solubility, and improving storage rate.
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Embodiment 1
[0029] Take 1 kg of dried August melon, crush it, immerse the August melon powder in 5L water and decoct twice, each time for 2 hours, separate the liquid residue, combine the filtrate, and use non-polar resin D101 (or neutral resin LSA- 21) Carry out column chromatography, eluted with water, the chromatography speed is 1L / h, discard the off-column water and wash 2L off-column liquid (containing monosaccharide and small molecular weight impurities), and then receive the water eluate (polysaccharide and flavonoid Feed liquid) 10L, then carry out membrane concentration to the water eluent, and dry the concentrated solution at a drying temperature of 60°C to finally obtain a light brown powdery product.
[0030] After weighing, 437g of the product was obtained, with a yield of 43.7%.
[0031] After testing, the content of total flavonoids in the product is 7.1wt%.
Embodiment 2
[0033] Take 1kg of dried August melon, crush it, add 5g of cellulase, 12g of α-amylase and 10L of water, stir and extract at 40°C for 0.5h, and then use non-polar resin D101 (or neutral resin LSA-21) for extraction Column chromatography, water elution, chromatography speed 1L / h, discard the upper water feed liquid and the 2L column liquid before water washing (containing monosaccharides and small molecular weight impurities)), and then receive the water eluate (polysaccharides and flavonoids Feed liquid) 10L, then carry out membrane concentration to water eluent, and carry out drying to concentrated solution, drying temperature is 60 ℃, finally obtains the powdery product of pale brown color.
[0034] After weighing, 679g of the product was obtained, with a yield of 67.9%.
[0035] After testing, the content of total flavonoids in the product is 11.3wt%.
Embodiment 3
[0037] Take 1kg of dried August melon, crush it, add 5g of cellulase, 12g of α-amylase and 5L of water, stir and extract at 40°C for 0.5h, then add 20g of pectinase, stir at 40°C for 10min, then let stand for 24h , and then filter to remove the precipitate, take the supernatant, use membrane T-10 (or membrane T-7) for clarification and filtration, then use membrane G20 (or membrane G19) to separate polysaccharides and flavonoids from the filtrate, and keep the solution in the membrane The solution is dried at a temperature of 60° C. to obtain a light brown powdery product.
[0038] After weighing, 467g of the product was obtained, with a yield of 46.7%.
[0039] After testing, the content of total flavonoids in the product is wtw13.0%.
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