Method for extracting flavone from holboellia latifolia

A flavonoid and powder technology, which is applied in the field of plant active ingredient extraction, can solve the problems of peculiar smell of gum, high energy consumption, limited application, etc., and achieves the effects of increasing total flavonoid content, good water solubility, and improving storage rate.

Inactive Publication Date: 2020-07-10
汪守鹏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] 1. Both n-butanol and acetic acid are high-boiling-point solvents, which require high-temperature drying and consume a lot of energy;
[0010] 2. During the high-temperature drying process, there will be gum odor and poor product quality;
[0011] 3. The content of flavonoids in the product is about 7%, which is relatively low and needs to be further improved;
[0012] 4. The product not only has poor water solubility, but also has chemical solvent residues, which limits the application and the market cannot be expanded

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Take 1 kg of dried August melon, crush it, immerse the August melon powder in 5L water and decoct twice, each time for 2 hours, separate the liquid residue, combine the filtrate, and use non-polar resin D101 (or neutral resin LSA- 21) Carry out column chromatography, eluted with water, the chromatography speed is 1L / h, discard the off-column water and wash 2L off-column liquid (containing monosaccharide and small molecular weight impurities), and then receive the water eluate (polysaccharide and flavonoid Feed liquid) 10L, then carry out membrane concentration to the water eluent, and dry the concentrated solution at a drying temperature of 60°C to finally obtain a light brown powdery product.

[0030] After weighing, 437g of the product was obtained, with a yield of 43.7%.

[0031] After testing, the content of total flavonoids in the product is 7.1wt%.

Embodiment 2

[0033] Take 1kg of dried August melon, crush it, add 5g of cellulase, 12g of α-amylase and 10L of water, stir and extract at 40°C for 0.5h, and then use non-polar resin D101 (or neutral resin LSA-21) for extraction Column chromatography, water elution, chromatography speed 1L / h, discard the upper water feed liquid and the 2L column liquid before water washing (containing monosaccharides and small molecular weight impurities)), and then receive the water eluate (polysaccharides and flavonoids Feed liquid) 10L, then carry out membrane concentration to water eluent, and carry out drying to concentrated solution, drying temperature is 60 ℃, finally obtains the powdery product of pale brown color.

[0034] After weighing, 679g of the product was obtained, with a yield of 67.9%.

[0035] After testing, the content of total flavonoids in the product is 11.3wt%.

Embodiment 3

[0037] Take 1kg of dried August melon, crush it, add 5g of cellulase, 12g of α-amylase and 5L of water, stir and extract at 40°C for 0.5h, then add 20g of pectinase, stir at 40°C for 10min, then let stand for 24h , and then filter to remove the precipitate, take the supernatant, use membrane T-10 (or membrane T-7) for clarification and filtration, then use membrane G20 (or membrane G19) to separate polysaccharides and flavonoids from the filtrate, and keep the solution in the membrane The solution is dried at a temperature of 60° C. to obtain a light brown powdery product.

[0038] After weighing, 467g of the product was obtained, with a yield of 46.7%.

[0039] After testing, the content of total flavonoids in the product is wtw13.0%.

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PUM

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Abstract

The invention discloses a method for extracting flavone from holboellia latifolia. The method comprises the following steps: adding cellulase, alpha-amylase and water into a holboellia latifolia powder, performing stirring and extracting for 0.5 h at 50-70 DEG C, then adding pectinase, performing stirring for 10 min at 50 DEG C, then performing standing for 24 h, performing filtering to remove precipitate, taking supernatant, then clarifying and filtering the supernatant, separating polysaccharide and flavone in filtrate, and leaving and drying a flavone feed liquid to finally obtain a light brown powdery product. The method has the beneficial effects that the holboellia latifolia flavone extracted by adopting the method has no peculiar smell, no chemical solvent residue, good water solubility and high content, can be applied to production of cosmetics, foods, medicines and the like, and has a greatly expanded application range and a better market prospect.

Description

technical field [0001] The invention relates to a method for extracting flavonoids, in particular to a method for extracting flavonoids from August melon, and belongs to the technical field of plant active ingredient extraction. Background technique [0002] August melon is a kind of wild fruit, which mainly grows in some mountainous areas of Hunan and Hubei in my country, and is also distributed in central and southern China, eastern China, Sichuan and Chongqing, Yunnan, Shaanxi, Gansu and other places. [0003] August melon fruit looks like a banana, with juicy pulp and sweet fruity taste. It is not only rich in vitamins and amino acids (including valine acid, methionine, isoleucine, phenylalanine, lysine, etc. that cannot be synthesized by the human body) , soluble fructose, starch, carbohydrates, organic acid protein and flavonoids, also rich in elements such as calcium, phosphorus, iron, zinc, selenium and various trace elements needed by the human body. It is called "w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K8/9789A23L33/105
CPCA23V2002/00A61K36/185A61K2236/19A61K2236/331A61K2236/51A61K2236/53A23L33/105A61K8/9789A23V2200/30A23V2250/2116
Inventor 汪守鹏
Owner 汪守鹏
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