A Simple Method to Enhance the Contrast of Micro-CT Plant Samples

A technology of contrast and plants, applied in the biological field, can solve problems such as not widely used in research, uniform internal density, and difficult structure edges

Active Publication Date: 2021-12-03
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the late 1990s, micro-CT began to be applied to plant research, to study root structure, morphological development and plant fossils, etc., but in general, the research application in plants is still not widespread
In addition, some samples have a uniform internal density
These lead to no significant difference in ray transmittance, and it is very difficult to distinguish the edges of sample structures with similar densities. The reconstructed image has very little contrast and high noise (Rousseau et al., 2015)

Method used

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  • A Simple Method to Enhance the Contrast of Micro-CT Plant Samples
  • A Simple Method to Enhance the Contrast of Micro-CT Plant Samples
  • A Simple Method to Enhance the Contrast of Micro-CT Plant Samples

Examples

Experimental program
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Embodiment Construction

[0033] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0034] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0035] Dissolve phosphotungstic acid in FAA fixative solution to obtain a phosphotungstic acid solution with a mass concentration of 1-20%, wherein the composition of FAA fixative solution is as follows: FAA fixative solution (100ml): 90ml of 50% ethanol aqueous solution, 5ml of glacial acetic acid , formaldehyde 5ml.

[0036] 1. Put pepper seeds into FAA fixative solution, 1%, 10% and 20% phosphotungstic acid solution respectively, turn it upside down several times to remove air bubbles, until the sample sinks into the solution, dye and fix at 4°C for 8 days, and then use Wash once with 2ml of 70% ethanol aqueous solution, put into the liquid tert-butanol in the centrifuge tube, shake three times to make the tert-butanol fully...

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Abstract

The invention discloses a simple method for enhancing the contrast of micro-CT plant samples. The method comprises the following steps: 1) dissolving phosphotungstic acid in FAA fixative solution to obtain a phosphotungstic acid solution; 2) using the phosphotungstic acid solution to soak the plant parts to be tested for fixation and dyeing; 3) using ethanol Washing the plant parts fixed and dyed in step 2), then adding to liquid tert-butanol and treating at -20°C, and then freeze-drying can enhance the contrast of the micro-CT of plant samples. The freeze-dried sample was scanned by micro-CT (Skyscan1172, Bruker Company), the scanning voltage can be 43V-49V, and the resolution can be 0.56-0.68μm. Parts of plant samples are greatly improved in contrast.

Description

technical field [0001] The invention relates to a simple method for enhancing the contrast of micro-CT plant samples, which belongs to the field of biotechnology. Background technique [0002] Micro-CT technology has been born for more than 40 years, and it has played a huge role in medicine, and has many applications in geology and materials science. In the late 1990s, micro-CT began to be used in plant research to study root structure, morphological development, and plant fossils, but generally speaking, it is still not widely used in plant research. [0003] Micro-CT imaging requires X-rays, while X-ray contrast imaging relies on the density, thickness, and atomic number of the sample (Lusic and Grinstaff, 2013). Since plant tissues are mainly composed of light elements, the X-ray absorption rate is low (about 20-40%), and the imaging effect of samples with an absorption rate of about 65-90% is the best. In addition, some samples have a uniform internal density. These ...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N23/046
CPCG01N1/30G01N23/046
Inventor 徐秀苹冯旻靳婉青梁荣花
Owner INST OF BOTANY CHINESE ACAD OF SCI
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