A Simple Method to Enhance the Contrast of Micro-CT Plant Samples
A technology of contrast and plants, applied in the biological field, can solve problems such as not widely used in research, uniform internal density, and difficult structure edges
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[0033] The experimental methods used in the following examples are conventional methods unless otherwise specified.
[0034] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.
[0035] Dissolve phosphotungstic acid in FAA fixative solution to obtain a phosphotungstic acid solution with a mass concentration of 1-20%, wherein the composition of FAA fixative solution is as follows: FAA fixative solution (100ml): 90ml of 50% ethanol aqueous solution, 5ml of glacial acetic acid , formaldehyde 5ml.
[0036] 1. Put pepper seeds into FAA fixative solution, 1%, 10% and 20% phosphotungstic acid solution respectively, turn it upside down several times to remove air bubbles, until the sample sinks into the solution, dye and fix at 4°C for 8 days, and then use Wash once with 2ml of 70% ethanol aqueous solution, put into the liquid tert-butanol in the centrifuge tube, shake three times to make the tert-butanol fully...
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