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Preparation method of electrochemical sensor for simultaneously detecting heavy metals Hg < 2 + > and Pb < 2 + >

An electrochemical and sensor technology, applied in the field of electrochemical sensor preparation, to achieve fast response, simple operation and good repeatability

Active Publication Date: 2020-07-10
HENAN UNIVERSITY OF TECHNOLOGY
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  • Abstract
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Problems solved by technology

At present, gold nanoflowers / functionalized carbon nanocomposites are used as electrode modification materials, and at the same time, the reduction of the signal molecule response signal at the 3' end of DNA-1 indicates the Hg in the sample. 2+ The content and the increase of metal-organic framework@bimetal / DNA-4 probe chain response signal indicate Pb in the sample 2+ The content is used for the heavy metal Hg in the sample 2+ and Pb 2+ The electrochemical biosensor method for simultaneous detection has not been reported

Method used

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Experimental program
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Embodiment 1

[0025] Specific steps are as follows:

[0026] (1) Preparation of gold nanoflowers / functionalized carbon nanocomposites: A gold nanoflower solution was prepared by in situ growth method and citrate reduction method, and 2 mL of 0.2 mM HAuCl was measured 4 Aqueous solution, adjusted to pH 7 with alkaline solution, added 0.02 mL of 20 mM NH 2 OH·HCl solution, then at 25°C, pipette 0.2 mL of 0.2 mM HAuCl 4 and 3 mL of 6% sodium citrate solution were added to the above mixed solution, and stood still for 2.5 h until the above mixed solution turned light pink to obtain the gold nanoflower solution. The carbon nanomaterials were treated by hydrothermal synthesis, and weighed Add 15 mg of carbon nanomaterials into 15 mL of distilled water, sonicate for 2 h, centrifuge at 8000 rpm for 6 min, and add the treated carbon nanomaterials into 75 μL of cationic polymer solution under the action of stirring. The solution was further reacted with 75 μL reducing solution at 90 °C for 24 h, an...

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Abstract

The invention relates to a preparation method of an electrochemical sensor for simultaneously detecting heavy metals Hg < 2 + > and Pb < 2 + >. The preparation method comprises the following steps: preparing a gold nanoflower / functionalized carbon nano composite material by an in-situ growth method and a hydrothermal synthesis method; preparing a metal organic framework-bimetallic material by an in-situ growth method; constructing a nucleic acid aptamer / gold nanoflower / functionalized carbon nano composite material / gold electrode by adopting a covalent bonding mode; using a platinum wire electrode as a counter electrode and a saturated silver chloride as a reference electrode and indicating the contents of Hg < 2 + > and Pb < 2 + > in a sample through reduction of a signal molecule responsesignal carried by the 3'end of the DNA-1 and increase of a metal organic framework-bimetallic / DNA-4 probe chain response signal so that the electrochemical sensor for simultaneously detecting the heavy metals Hg < 2 + > and Pb < 2 + > is obtained. Compared with other material modified gold electrodes and research means, the prepared novel electrochemical sensor has the advantages of being high inresponse speed, easy to operate, high in sensitivity, good in repeatability and high in accuracy.

Description

technical field [0001] The invention relates to a method for heavy metal Hg 2+ and Pb 2+ The preparation method of an electrochemical sensor for simultaneous detection particularly relates to a preparation method based on nucleic acid aptamer / gold nanoflower / functionalized carbon nanocomposite material / gold electrode. Background technique [0002] Human beings are very dependent on water sources, because water can sustain life and is the most basic guarantee. Water sources in many countries in the world are quite scarce. Although many national policies are constantly speeding up the solution to this situation, it is still difficult to solve the shortage of water sources in our country. In recent years, various industries in our country have developed rapidly. Among them, the development of large-scale new industries has caused a large amount of industrial wastewater discharge, which has made the already scarce water source even scarcer. Due to the discharge of a large amou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 何保山刘慧谢玲玲曹晓雨王晓曦郑学玲卫敏赵文红金华丽任文洁吴立根王涛
Owner HENAN UNIVERSITY OF TECHNOLOGY