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A method for tissue culture and rapid propagation of Quercus fern seedlings via somatic embryo pathway

A tissue culture rapid propagation and somatic cell technology, applied in the field of plant tissue culture, can solve the problems of unguaranteed spore quantity, mixed spores of different species, not very suitable for large-scale production of Quercus fern seedlings, etc.

Active Publication Date: 2021-09-21
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Affected by the amount and vitality of spores, if you want to produce every year, you need a nursery that specializes in cultivating adult plants to harvest spores. Otherwise, the amount of spores cannot be guaranteed, and there may be a risk of mixing different species of spores in field collection. It is not very suitable for Quercus fern seedlings mass production of
[0005] The tissue culture system of the patent "A Rapid Propagation Method of Quercus fern" (CN103430853A) reports the acquisition of callus and the induction of tetraploid, but since the callus is prone to variation, if the method of callus proliferation and differentiation is adopted, There is a certain risk for the large-scale artificial propagation of oak fern

Method used

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  • A method for tissue culture and rapid propagation of Quercus fern seedlings via somatic embryo pathway
  • A method for tissue culture and rapid propagation of Quercus fern seedlings via somatic embryo pathway

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Experimental program
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Effect test

Embodiment 1B

[0028] The effect of embodiment 1BA pretreatment on somatic embryogenesis of Quercus fern

[0029] The young spore leaves produced by the in vitro germination of Quercus fern spores were inoculated in the pretreatment medium containing different concentrations of 6-BA (0, 0.5, 1.0, 2.0, 3.0 mg / L). After continuing to culture for 60 days in the basic medium of hormones, observe and count the status of somatic embryos produced by the explants under a stereomicroscope.

[0030] Statistics on the efficiency of explants producing somatic embryos: After the explants were cultured in 1 / 2MS medium for 60 days, observe and count the number of somatic embryos produced on each explant under a stereomicroscope, calculate the production ratio, and produce Ratio=(number of explants producing n somatic embryos / number of explants producing somatic embryos)×100%, n is >30, 10-20, <10.

[0031] Statistics show that each treatment can make explants produce somatic embryos, and the concentration...

Embodiment 2

[0032] The effect of embodiment 2 sucrose concentration on somatic embryogenesis of Quercus fern

[0033] The juvenile spore leaves produced by the in vitro germination of Quercus fern spores were inoculated in the pretreatment medium containing 6-BA 2.0 mg / L, and after pre-cultivation for 7 days, they were transferred to culture medium containing different concentrations of sucrose (1%, 2%, 3 %, 4%, 5%) of the basic medium, after continuing to culture for 60 days, observe and count the status of the explants producing somatic embryos under a stereomicroscope.

[0034] Statistics on the efficiency of explants to produce somatic embryos: After the explants were cultured in 1 / 2MS medium for 60 days, observe and count the number of somatic embryos produced on each explant under a stereomicroscope, calculate the production ratio, and produce Ratio=(number of explants producing n somatic embryos / number of explants producing somatic embryos)×100%, n is >30, 10-20, <10.

[0035]Stat...

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Abstract

The invention provides a method for tissue culture and rapid propagation of Quercus fern seedlings via the somatic embryo pathway, comprising the following steps: obtaining explants; pre-cultivating the explants; subculture the explants; separating and inoculating the somatic embryos . The method of the invention can quickly obtain the somatic embryo of the oak fern, promote the formation of seedlings, improve the reproduction efficiency, and play an important role in promoting the protection, development and utilization of the wild oak fern resources.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for rapid propagation of oak fern tissue culture based on a somatic embryo approach. Background technique [0002] Oak fern (Drynaria roosii), also known as Shiyan ginger, Yanlian ginger, and monkey ginger, is a medium-sized epiphytic fern belonging to the genus Oak fern in the family Drynariaceae. In my country, it is mainly distributed in Zhejiang, Fujian, Taiwan, Guangdong, Guangxi, Jiangxi, Hubei, Sichuan, Guizhou, Yunnan and other places. Quercus fern is a medium-sized epiphytic fern. Drynaria rhizome is used as medicine in its rhizome. As the authentic product of drynaria, it was recorded in "Chinese Pharmacopoeia" (Zhou Tongshui and Zhou Ronghan, 1998). Studies have found that the content of naringin and total flavonoids in the rhizome of Quercus fern is higher than that of other similar plants, and it is the main active ingredient in Rhi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 石雷王頔
Owner INST OF BOTANY CHINESE ACAD OF SCI
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