Method for detecting GPC3 based on RGO-CS-Fc/Pt-Pd NPs nano composite material

A nanocomposite material, rgo-cs-fc technology, applied in the analysis of materials, material electrochemical variables, material analysis through electromagnetic means, etc., can solve the problems of expensive instruments, complicated operations, time-consuming technical requirements, etc., and achieve strong affinity , small size, low background interference effect

Active Publication Date: 2020-07-14
GUILIN UNIV OF ELECTRONIC TECH
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Problems solved by technology

The instruments used in these methods are expensive, complicated to operate, time-consuming and require high technical requirements, so it is necessary to establish a fast and easy-to-operate GPC3 detection method

Method used

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  • Method for detecting GPC3 based on RGO-CS-Fc/Pt-Pd NPs nano composite material
  • Method for detecting GPC3 based on RGO-CS-Fc/Pt-Pd NPs nano composite material
  • Method for detecting GPC3 based on RGO-CS-Fc/Pt-Pd NPs nano composite material

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Embodiment Construction

[0043] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0044] A method for detecting GPC3 based on RGO-CS-Fc / Pt-Pd NPs nanocomposite material, the detection principle see figure 1 . First, metal platinum and palladium are deposited on the surface of the activated screen-printed electrode. Then the RGO-CS-Fc composite material was added dropwise on the electrode surface, and the composite material would be fixed on the electrode surface through adsorption and chemical cross-linking. Then, by incubating the GPC3 aptamer, the aminated GPC3 aptamer can covalently bind to the RGO-CS-Fc on the surface of the electrode under the action of EDC / NHS, and because of its relatively unstable spatial structure, it can be used as a single The chain form is connected to the composite material, which in turn can be modified onto the electrode. After the GPC3 protein is added dropwise, the GPC3 aptamer will spe...

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Abstract

A liver cancer marker phosphatidylinositol proteoglycan 3 (Glypican-3, GPC3) is used as a research object, a GPC3 aptamer is used as a recognition probe, on the basis of good electron transfer effectand excellent loading capacity of a reductive graphene oxide-chitosan-ferrocene/nano platinum and palladium (Pt-Pd NPs/RGO-CS-Fc) composite material, the GPC3 aptamer can specifically recognize and becombined with GPC3 protein, and a novel aptamer sensor capable of performing specific recognition and quantitative analysis on the GPC3 protein is constructed and used for detecting the content of GPC3 in serum. The method is simple to operate, time-saving, low in cost and relatively low in detection limit.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a method for detecting glypican-3 (GPC3) based on a nanocomposite material combined with a suitable ligand. Background technique [0002] Primary hepatocellular carcinoma (HCC) is a malignant tumor that is relatively common in my country and can easily threaten people's lives. Common liver cancer markers include AFP, GPC3, GP73, etc. GPC3 exists in a very low concentration in normal human tissues, but the concentration in liver cancer patients is very high, so GPC3 is closely related to the occurrence and development of liver cancer. GPC3 detection methods mainly include radioimmunoassay, fluorescence immunoassay, enzyme-linked immunosorbent assay, chemiluminescence immunoassay, flow immunoassay, electrochemical immunosensor, piezoelectric immunosensor, etc. The patent for invention with publication number CN 105717104 B relates to a method of using a membrane fi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3278G01N27/48
Inventor 李桂银史校行李玉秋周治德梁晋涛
Owner GUILIN UNIV OF ELECTRONIC TECH
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