Method for detecting GPC3 based on RGO-CS-Fc/Pt-Pd NPs nano composite material

A nanocomposite material, rgo-cs-fc technology, applied in the analysis of materials, material electrochemical variables, material analysis through electromagnetic means, etc., can solve the problems of expensive instruments, complicated operations, time-consuming technical requirements, etc., and achieve strong affinity , small size, low background interference effect
CN111413385AActive Publication Date: 2020-07-14GUILIN UNIV OF ELECTRONIC TECH
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
GUILIN UNIV OF ELECTRONIC TECH
Publication Date
2020-07-14

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Abstract

A liver cancer marker phosphatidylinositol proteoglycan 3 (Glypican-3, GPC3) is used as a research object, a GPC3 aptamer is used as a recognition probe, on the basis of good electron transfer effectand excellent loading capacity of a reductive graphene oxide-chitosan-ferrocene / nano platinum and palladium (Pt-Pd NPs / RGO-CS-Fc) composite material, the GPC3 aptamer can specifically recognize and becombined with GPC3 protein, and a novel aptamer sensor capable of performing specific recognition and quantitative analysis on the GPC3 protein is constructed and used for detecting the content of GPC3 in serum. The method is simple to operate, time-saving, low in cost and relatively low in detection limit.
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Description

technical field

[0001] The invention belongs to the field of biological detection, and in particular relates to a method for detecting glypican-3 (GPC3) based on a nanocomposite material combined with a suitable ligand. Background technique

[0002] Primary hepatocellular carcinoma (HCC) is a malignant tumor that is relatively common in my country and can easily threaten people's lives. Common liver cancer markers include AFP, GPC3, GP73, etc. GPC3 exists in a very low concentration in normal human tissues, but the concentration in liver cancer patients is very high, so GPC3 is closely related to the occurrence and development of liver cancer. GPC3 detection methods mainly include radioimmunoassay, fluorescence immunoassay, enzyme-linked immunosorbent assay, chemiluminescence immunoassay, flow immunoassay, electrochemical immunosensor, piezoelectric immunosensor, etc. The patent for invention with publication number CN 105717104 B relates to a method of using a membrane fi...

Claims

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