A method for non-destructive inoculation of black spot fungus in sweet potato pieces

A technology of black spot bacteria and potato pieces, which is applied in the field of agricultural plant protection plant pathogen inoculation, can solve the problems of large-scale infection, infection of potato pieces, and potato piece wound inoculation, etc., so as to fill technical gaps, avoid potato pieces wounds, and ensure integrity. sexual effect

Active Publication Date: 2021-12-28
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can inject the spore suspension by acupuncture at the specific position of the sweet potato, and can accurately determine the shape and radius of the black spot, but this method has obvious deficiencies: (1) The wound of the potato block is artificially caused and goes deep into the dermis , in the identification of disease resistance of potato cubes, the effect of different varieties of potato cubes, potato skins and subcutaneous tissue on resisting pathogen invasion cannot be evaluated; The infestation process of the tuber skin, then attaching to the tuber surface, and then invading the tuber tuber is different
As a result, when screening fungicides for the prevention and treatment of tuber black spot and studying the development of disease in the process of susceptible tuber and tuber emergence, it is impossible to completely simulate the natural disease, which has a greater interference with the test results; (3) artificially causing tuber tuber Wound inoculation, during the process of moisturizing or growing seedlings, bacteria will infect the potato pieces through the wound, causing the potato pieces to rot or infection in a large area, interfering with the test results, or even failing to obtain the test results
Therefore, although the acupuncture method can guarantee the occurrence of black spot on the acupuncture point of sweet potato pieces, it is completely unable to simulate the disease process in the natural state.

Method used

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  • A method for non-destructive inoculation of black spot fungus in sweet potato pieces
  • A method for non-destructive inoculation of black spot fungus in sweet potato pieces
  • A method for non-destructive inoculation of black spot fungus in sweet potato pieces

Examples

Experimental program
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Effect test

Embodiment 1

[0052] According to the above-mentioned experimental materials and inoculation methods, 90 identical sweet potato pieces were divided into 3 groups, 30 pieces in each group; 6 spores / mL; the final inoculation time at 20-30°C was 24h, 48h, and 72h;

[0053] After each group was inoculated, it was taken out to dry, and each group of 10 pieces was used as a repetition, and placed in a 25°C incubator for moisture cultivation. Calculated from the date of inoculation, the number of diseased spots on potato tubers was investigated 6 days later, and the results are shown in Table 1.

[0054] Table 1 The incidence of sweet potato tubers at different inoculation times

[0055]

[0056]

[0057] Note: Different lowercase letters after the data in the same column indicate significant differences at the 0.05 level, and different uppercase letters indicate significant differences at the 0.01 level.

[0058] According to the test results in Table 1, the planting time was 24h, 48h, an...

Embodiment 2

[0060] According to the above-mentioned experimental materials and inoculation methods, 120 identical sweet potato pieces were taken and divided into 4 groups, 30 pieces in each group; the concentration of the spore liquid used in each group was 2.5×10 5 spores / mL, 5×10 5 spores / mL, 7.5×10 5 spores / mL, 1×10 6 spores / mL; the final inoculation time at 20-30°C was 48 hours;

[0061] After the inoculation of each group was completed, each group of 10 pieces was used as a repetition, and placed in a 25°C incubator for moisture cultivation. Calculated from the date of inoculation, the number of diseased spots on potato tubers was investigated 6 days later, and the results are shown in Table 2.

[0062] Table 2 Disease incidence of inoculated potato pieces under different inoculation intensities

[0063]

[0064] Note: Different lowercase letters after the data in the same column indicate significant differences at the 0.05 level, and different uppercase letters indicate signi...

Embodiment 3

[0068] According to the above-mentioned experimental materials and inoculation methods, 540 identical sweet potato pieces were divided into 6 groups, 90 pieces in each group; the concentration of the spore suspension used in each group was 7.5×10 5 spores / mL; the final inoculation time at 20-30°C was 48 hours;

[0069] After the inoculation was completed, the potato pieces were dried in the air, and the excess inoculation carrier on the surface of the potato pieces was gently shaken off, and the potato soaking treatment was repeated (each group was: 30 pieces of sweet potatoes were repeated 3 times), and the potato soaking was repeated three times. The time is 10 minutes.

[0070] The 6 groups of medicament-soaked potatoes are as follows: a blank control (no medicament treatment, using clear water), the other 5 groups are specifically the active ingredient dosages of SY01 are 250mg / kg, 333mg / kg, 500mg / kg respectively, and the active ingredient dosages of iprodione 475mg / kg, t...

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Abstract

The invention provides a method for non-destructive inoculation of black spot fungus in sweet potato cubes. Specifically, the intact sweet potato cubes are placed in a mixture of pathogen spore suspension and inoculation carrier to inoculate potato cubes, and the inoculation management is carried out in a closed environment . The concentration of the pathogen spore suspension is not more than 10 6 spores / mL, the volume ratio of the carrier to the pathogen spore suspension is 6.5:1, and the inoculation time does not exceed 72 hours. This inoculation method does not damage the potato skin, is very similar to the actual growth and storage conditions of the potato cubes, and simulates the natural process of the black spot virus infecting the potato cubes to the greatest extent. It avoids the potato piece wound caused by acupuncture potato piece inoculation, avoids the problem that the potato piece is easy to rot and cannot be tested and identified due to the rot in the later test or identification process, and makes it possible to study the resistance of the potato piece skin to black spot disease. It is possible to ensure the integrity of potato pieces infected with black spot disease during the test, so that the infected potato pieces can be effectively followed up in the test, which has important application value.

Description

technical field [0001] The invention belongs to the inoculation technology of pathogenic bacteria for agricultural plant protection, and in particular relates to a method for inoculating sweet potato pieces with black spot pathogen without damage. Background technique [0002] Sweet potato black spot disease occurs in all sweet potato producing areas in the world, and the annual loss of sweet potato production caused by the disease reaches 5% to 10%, and when the damage is serious, the loss can reach 20% to 50%, or even higher. In addition, diseased potatoes can produce toxic substances such as sweet potato ipomeamarone, which can cause poisoning and death in severe cases. The disease is caused by Ceratocystis fimbriata Ellis et Halsted infecting sweet potatoes, and can be transmitted through various channels such as seed potatoes, seedlings and soil, and it is difficult to completely eradicate. [0003] At present, in production, the occurrence of sweet potato black spot i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/06A01G31/00A01G24/12A01G24/15
CPCA01G7/06A01G31/00A01G24/15A01G24/12
Inventor 张德胜张振臣乔奇王永江王爽田雨婷白瑞英赵付枚
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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