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A method for preserving adipose tissue, adipose tissue preservation solution and preparation method thereof

A technology of adipose tissue and preservation methods, which is applied in the preservation, application, and animal husbandry of human or animal bodies. It can solve the problems of complex components of the preservation solution, inconvenient application, and cumbersome preparation process, so as to avoid quality differences and maintain Vibrant, low-cost results

Active Publication Date: 2021-05-18
GUANGDONG CHINAHEALTH LIFE SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the composition of the preservation solution is complicated, and the preparation process is cumbersome, so it cannot be applied conveniently.

Method used

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  • A method for preserving adipose tissue, adipose tissue preservation solution and preparation method thereof
  • A method for preserving adipose tissue, adipose tissue preservation solution and preparation method thereof
  • A method for preserving adipose tissue, adipose tissue preservation solution and preparation method thereof

Examples

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Embodiment 1

[0038] This embodiment is an adipose tissue preservation solution of the present invention, which contains 5% (v / v) human serum albumin, 1× norfofloxacin, 1% (v / v) dextran- 40 and 5 ng / mL Ac-DEVD-CHO in polyethylene glycol 400.

[0039]The preparation method of the above-mentioned adipose tissue preservation solution is: add 20% (v / v) human serum albumin, 100 × Norfofloxacin, 6% (v / v) dextran-40 and Ac-DEVD-CHO, so that the content of each component is as follows: 5% (v / v) human albumin, 1 × Norfofloxacin, 1% (v / v) dextran-40 and 5ng / mL Ac-DEVD -CHO, the adipose tissue preservation solution can be prepared. Wherein, the preparation method of the 5-20ng / mL Ac-DEVD-CHO is: weigh the Ac-DEVD-CHO, dissolve it in polyethylene glycol 400, obtain a 5ng / mL Ac-DEVD-CHO solution, and filter it at 0.22 μm , the 5ng / mL Ac-DEVD-CHO can be prepared.

Embodiment 2

[0041] This embodiment is an adipose tissue preservation solution of the present invention, which contains 5% (v / v) human serum albumin, 1× norfofloxacin, 1% (v / v) dextran- 40 and 20 ng / mL Ac-DEVD-CHO in polyethylene glycol 400.

[0042] The preparation method of the above-mentioned adipose tissue preservation solution is: add 20% (v / v) human serum albumin, 100 × Norfofloxacin, 6% (v / v) dextran-40 and Ac-DEVD-CHO, so that the content of each component is as follows: 5% (v / v) human albumin, 1 × Norfofloxacin, 1% (v / v) dextran-40 and 20ng / mL Ac-DEVD -CHO, the adipose tissue preservation solution can be prepared. Wherein, the preparation method of the 5-20ng / mL Ac-DEVD-CHO is as follows: Ac-DEVD-CHO is weighed, dissolved in polyethylene glycol 400 to obtain a 20ng / mL Ac-DEVD-CHO solution, and filtered at 0.22 μm , the 20ng / mL Ac-DEVD-CHO can be prepared.

Embodiment 3

[0044] This embodiment is an adipose tissue preservation solution of the present invention, which contains 5% (v / v) human serum albumin, 1× norfofloxacin, 1% (v / v) dextran- 40 and 10 ng / mL Ac-DEVD-CHO in polyethylene glycol 400.

[0045] The preparation method of the above-mentioned adipose tissue preservation solution is: add 20% (v / v) human serum albumin, 100 × Norfofloxacin, 6% (v / v) dextran-40 and Ac-DEVD-CHO, so that the content of each component is as follows: 5% (v / v) human albumin, 1 × Norfofloxacin, 1% (v / v) dextran-40 and 15ng / mL Ac-DEVD -CHO, the adipose tissue preservation solution can be prepared. Wherein, the preparation method of the 5-20ng / mL Ac-DEVD-CHO is as follows: Ac-DEVD-CHO is weighed, dissolved in polyethylene glycol 400 to obtain a 15ng / mL Ac-DEVD-CHO solution, and filtered at 0.22 μm , the 15ng / mL Ac-DEVD-CHO can be prepared.

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Abstract

The invention relates to a preservation method of adipose tissue, a preservation solution of adipose tissue and a preparation method thereof, and belongs to the field of preservation of biological isolated tissues or organs. The adipose tissue preservation solution provided by the present invention includes polyethylene glycol 400, nutrients, antibacterial agents, dextran-40 and apoptosis inhibitors, wherein the polyethylene glycol 400 contains a final concentration of 5% (v / v ), 1× norfofloxacin, dextran‑40 at a final concentration of 1% (v / v), and Ac‑DEVD‑CHO at a final concentration of 5‑20 ng / mL. The adipose tissue preservation solution provided by the present invention has low cost, stable and safe components, no toxic and side effects, and can be used for long-term preservation of isolated adipose tissue, and can maintain the original biological characteristics of adipose-derived mesenchymal stem cells within 72 hours. It can well maintain the viability of adipose-derived mesenchymal stem cells without causing functional variation of cells.

Description

technical field [0001] The invention relates to a preservation method of adipose tissue, a preservation solution of adipose tissue and a preparation method thereof, and belongs to the field of preservation of biological isolated tissues or organs. Background technique [0002] Adipose tissue is mainly composed of a large number of clustered fat cells separated into lobules by loose connective tissue. Adipose tissue is divided into two types based on the structure and function of fat cells. Yellow (white) adipose tissue is yellow (white in some mammals), which is commonly referred to as adipose tissue. It is formed by the accumulation of a large number of single-vessel fat cells. There is a large lipid droplet in the center of the cell, and the cytoplasm is in a thin layer. , located at the cell periphery, surrounding lipid droplets. On the HE section, the lipid droplet was dissolved into a large vacuole, the nucleus was oblate, pushed to the side of the cell by the lipid d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/02A01N1/0215A01N1/0221A01N1/0226
Inventor 李文东宋云庆黎波卢瑞珊
Owner GUANGDONG CHINAHEALTH LIFE SCI CO LTD
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