Anti-CLD18A2 nanobody and application thereof

A nanobody, FR1 technology, applied in the direction of antibody medical components, recombinant DNA technology, antibody mimics/scaffolds, etc.
CN111434692AActive Publication Date: 2020-07-21ZHEJIANG DOER BIOLOGICS CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG DOER BIOLOGICS CO LTD
Publication Date
2020-07-21

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Abstract

The invention relates to the field of biology, in particular to an anti-CLD18A2 nanobody. According to the anti-CLD18A2 nanobody provided by the invention, a complementary determining region (CDR) ofthe anti-CLD18A2 nanobody includes CDR1-CDR3 of which the amino acid sequences are shown as follows: CDR1 of which the amino acid sequence is shown by one of SEQ ID NO.4-11, CDR2 of which the amino acid sequence is shown by one of SEQ ID NO.19-26 and CDR3 of which the amino acid sequence is shown by one of SEQ ID NO.33-39. The anti-CLD18A2 nanobody provided by the invention can be specifically bound to an epitope present on CLD18A2, and fusion protein corresponding to the nanobody has good specificity and affinity on CLD18A2, and has an obvious tumor suppression effect.
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Description

technical field

[0001] The invention relates to the field of biology, in particular to an anti-CLD18A2 nanobody and an application thereof. Background technique

[0002] Claudin 18 (Claudin 18, CLD18) is a transmembrane protein with a molecular weight of about 28kD, located in the tight junction of epithelium and endothelium, and is tightly connected between adjacent cells. In normal epithelial tissue, claudin on the cell surface is difficult to access due to the tight intercellular space, while the intercellular space in tumor cells is relatively loose. Therefore, claudin on tumor cells has become a potential target for extracellular antibodies and immunotherapy. CLD18 has four hydrophobic regions, which form two extracellular domains as transmembrane regions, in which hydrophobic region 1 and hydrophobic region 2 surround to form extracellular domain 1, and hydrophobic region 3 and hydrophobic region 4 surround to form extracellular domain 2. Due to the different splicing...

Claims

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