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Reagent for antagonizing physiological action of n-butyl alcohol

A physiological effect, n-butanol technology, applied in biocides, plant growth regulators, plant growth regulators, etc., can solve the problem of not showing similar n-butanol treatment results

Inactive Publication Date: 2020-07-24
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Interestingly, however, it was found in the above studies that the two isomers of n-butanol (sec-butanol and tert-butanol) hardly exhibited similar treatment results as n-butanol in the above treatments.

Method used

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  • Reagent for antagonizing physiological action of n-butyl alcohol
  • Reagent for antagonizing physiological action of n-butyl alcohol
  • Reagent for antagonizing physiological action of n-butyl alcohol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] ABA synthesis inhibitor antagonizes the effect of n-butanol on Arabidopsis seed germination

[0034] n-Butanol inhibits seed germination and induces cotyledon deformation in Arabidopsis thaliana. Adding 1 μM, 10 μM, 20 μM MABA synthesis inhibitor Fluridone to the medium containing 0.1% n-butanol can improve the seed germination rate and cotyledon extension rate inhibited by n-butanol to a certain extent. Arabidopsis seeds were grown under light for 3 days on MS medium, medium containing 0.1% n-butanol, medium containing 0.1% n-butanol and Fluridone, and the effects on germination were as follows: figure 1 and figure 2 As shown, the germination rate of Arabidopsis seeds in MS culture is about 95%, while the seed germination rate is about 25% under the treatment of adding 0.1% n-butanol. Under the simultaneous treatment of 0.1% n-butanol and 10 μM Fluidone The germination rate of the seeds was about 80%, but the germination rate was not significantly improved by adding...

Embodiment 2

[0037] High concentration of gibberellin antagonizes the effect of n-butanol on seed germination of Arabidopsis thaliana

[0038]Adding 10μM, 30μM, 50μM, 100μM gibberellin to the medium containing 0.1% n-butanol at the same time can increase the seed germination rate and cotyledon extension rate under the inhibition of n-butanol in different degrees. Arabidopsis seeds were grown under light for 3 days on MS medium, medium containing 0.1% n-butanol, medium containing 0.1% n-butanol and different concentrations of gibberellin, and the results were as follows: Figure 4 and Figure 5 As shown, the germination rate of Arabidopsis seeds in MS culture is about 95%, while the seed germination rate is about 12% under the treatment of adding 0.1% n-butanol, while adding 0.1% n-butanol and 10μM gibberellin medium The germination rate of Arabidopsis thaliana seeds was about 26% under the treatment, and the germination rate of Arabidopsis thaliana seeds was about 40% when adding 0.1% n-b...

Embodiment 3

[0041] Gibberellin antagonizes the effect of n-butanol on inhibiting Arabidopsis taproot growth

[0042] Arabidopsis seeds were cultured in MS medium for 3 days, and then the seedlings were transferred to MS normal medium, medium containing 30 μM gibberellin, medium containing 0.2% n-butanol, medium containing 0.2% n-butanol and 30 μM On the medium containing 0.4% n-butanol, and on the medium containing 0.4% n-butanol and 30 μM gibberellin, the gibberellin culture medium continued vertically under light for 5 days. To measure the length of the main root Figure 7 As shown, the tap root length of seedlings grown on MS and culture containing 30 μM gibberellin was about 35 mm, on medium containing 0.2% n-butanol and on medium containing 0.2% n-butanol plus 30 μM gibberellin The tap root lengths of the seedlings were 22 mm and 31 mm, respectively, and the tap root lengths of the seedlings grown on the medium containing 0.2% n-butanol and the medium containing 0.2% n-butanol plus ...

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Abstract

The invention provides a reagent for antagonizing physiological action of n-butyl alcohol, and relates to the technical field of hormone function research, and the active component of the reagent comprises a synthesis inhibitor of abscisic acid or gibberellin. In the reagent, 10 [mu] M Fluridone can effectively antagonize the n-butyl alcohol to inhibit seed germination; the gibberellin can improvethe seed germination rate and cotyledon extension rate under inhibition of the n-butyl alcohol to different extents, the higher the concentration is, the more obvious the effect is, and the antagonistic effect of 30 [mu] M gibberellin is optimal; high-concentration GA (30 [mu] M) can recover the division ability of root tip stem cells to a great extent, and finally partially recovers the growth of main roots.

Description

technical field [0001] The invention belongs to the technical field of hormone function research, and in particular relates to a reagent for antagonizing the physiological action of n-butanol. Background technique [0002] Gibberellins (Gibberellins, GAs), diterpenoid phytohormones, mainly promote the vegetative growth of plants, including elongation of internodes, elongation of roots and expansion of leaves. The gibberellin signal transfers to the pathway, and the DELLA protein, as a plant growth inhibitor, can be counteracted by the active gibberellin. The gibberellin signal initiates the degradation of the DELLA protein, regulates the expression of gibberellin-responsive genes, and thus promotes plant growth. DELLA protein plays a negative feedback regulation on plant growth and development. [0003] Transphosphatidylation refers to that under the catalysis of phospholipase D (phospholipase D, PLD), the phosphodiester bond of glycerophospholipid is broken, and the phosph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/12A01N45/00A01N43/40A01N31/02A01N25/00A01P21/00
CPCA01N25/00A01N31/02A01N43/12A01N43/40A01N45/00
Inventor 贾艳霞李唯奇陈娟
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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