Lung cancer screening kit

A technology of kits and reagents, applied in the field of lung cancer screening kits, can solve problems such as the unrecognized relationship with ZNF808, and achieve low cost, less time-consuming, and effective detection

Inactive Publication Date: 2020-07-24
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Abstract
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  • Lung cancer screening kit
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experiment example Z

[0090] Experimental example The relationship between ZNF808 methylation level and lung cancer

[0091] 1. Clinical data

[0092] 56 patients with lung cancer and 44 patients with benign lung diseases were selected. The basic information is as follows:

[0093]

[0094] 2. ZNF808 methylation sequencing

[0095] (1) Lung tissue DNA extraction: DNA was extracted from tissue samples using the tissue DNA extraction and purification kit QlAampDNA Mini Kit (article number: 51304) purchased from Qiagen;

[0096] (2) DNA concentration measurement and quality inspection: Take 1 μl to measure the concentration of Qubit, and print the label to paste the barcode; take 1 μl for 2100 quality inspection;

[0097] (3) Bisμlfite (bisulfite) conversion: the Bisμlfite conversion experiment was carried out using the methylation kit EZDNA Methylation-Lightning Kit (Cat. No.: D5030T) from Zymo research;

[0098] (4) Library construction: use the Ironman library construction kit from Guangzhou ...

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Abstract

The invention provides a lung cancer screening kit, which is a kit for detecting ZNF808 gene methylation of lung tissue. The invention also provides application of a reagent for detecting ZNF808 genemethylation in preparation of a lung cancer screening reagent. The kit can be used for auxiliary diagnosis of clinical lung cancer, provides an effective basis for patients to take related treatment measures or make decisions and has a good clinical application prospect.

Description

technical field [0001] The invention relates to a screening kit for lung cancer. Background technique [0002] Lung cancer is one of the malignant tumors with the fastest increasing morbidity and mortality and the greatest threat to the health and life of the population. In the past 50 years, many countries have reported that the morbidity and mortality of lung cancer have increased significantly. The incidence and mortality of lung cancer in men rank first among all malignant tumors, and the incidence and mortality of lung cancer occupy the second place in women. Non-small cell lung cancer is a type of lung cancer, which accounts for about 80% of all lung cancers, and about 75% of patients are already in the middle and advanced stages when they are discovered, and the 5-year survival rate is very low. Therefore, early and rapid diagnosis of lung cancer is very important. [0003] Zinc finger proteins are a class of proteins that contain zinc fingers, which are short prote...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 李为民李镭王维雯
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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