Multifunctional ultrasonic contrast agent and preparation method thereof

An ultrasonic contrast agent and multifunctional technology, applied in the field of biomedicine, can solve the problems that multifunctional reagents/drugs cannot meet the needs, and achieve excellent in vivo near-infrared fluorescence imaging performance, enhanced imaging effect, and excellent PTT effect.

Inactive Publication Date: 2020-07-28
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is that the development of theranostics requires reagents / drugs with a variety of functions, and these different functions need to be effectively brought into play. Currently, the relevant multifunctional reagents / drugs are far from meeting the demand.

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  • Multifunctional ultrasonic contrast agent and preparation method thereof
  • Multifunctional ultrasonic contrast agent and preparation method thereof
  • Multifunctional ultrasonic contrast agent and preparation method thereof

Examples

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Effect test

Embodiment 1

[0048] A preparation method of the multifunctional ultrasonic contrast agent of the present invention, the steps are as follows:

[0049] (1) Dissolving the organic phase: Dissolve 100mg of poly(lactic acid-glycolic acid) copolymer in 3ml of dichloromethane, add 200ul of SPIO dispersion, 3mg of IR780 and stir until completely dissolved, then add 200ul of PFH to obtain a mixed solution.

[0050] (2) Emulsification: Add 15ml concentration to the mixed solution obtained after step (1) and be 4% polyvinyl alcohol solution, carry out ultrasonic emulsification to obtain emulsion, wherein, the power of ultrasonic emulsification is 110W, and the time of ultrasonic emulsification is 120s.

[0051] (3) Volatile organic phase: magnetically stir the emulsion obtained after step (2) for 2-3 hours to solidify the surface of the microspheres, dichloromethane volatilize as much as possible naturally, then centrifuge at 12000rpm for 7 minutes at high speed, take the precipitate and rinse it 3 t...

Embodiment 2

[0071] A kind of multifunctional ultrasonic contrast agent of the present invention, its preparation steps are as follows:

[0072] (1) Dissolving the organic phase: Dissolve 100mg poly(lactic acid-glycolic acid) copolymer in 3ml dichloromethane, add 200ul SPIO and 3mg IR780 in turn and stir until completely dissolved, then add 200ul PFH to obtain a mixed solution.

[0073] (2) Emulsification: Add 15ml concentration of 5% polyvinyl alcohol solution to the mixed solution obtained after step (1), and carry out ultrasonic emulsification to obtain emulsion; the power of ultrasonic emulsification is 110W, and the time of ultrasonic emulsification is 120s.

[0074] (3) Volatile organic phase: The emulsion obtained after step (2) is magnetically stirred for 2-3 hours to solidify the surface of the microspheres, dichloromethane is naturally volatilized as much as possible, and then centrifuged at a high speed of 13000rpm for 7 minutes, and the precipitate is rinsed After 3 times, the ...

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Abstract

The invention discloses a multifunctional ultrasonic contrast agent. The multifunctional ultrasonic contrast agent is of a core-shell structure, wherein a poly(lactic-co-glycolic acid) copolymer is used as a shell, perfluorohexane is used as a core, and superparamagnetic iron oxide and IR780 iodide are loaded in the poly(lactic-co-glycolic acid) copolymer. The invention also discloses a preparation method of the contrast agent. The preparation method comprises the following steps: (1) dissolving the poly(lactic-co-glycolic acid) copolymer, the superparamagnetic iron oxide, the IR780 iodide andthe perfluorohexane in an organic solvent to obtain a mixed solution; (2) adding a polyvinyl alcohol solution into the mixed solution obtained in the step (1), and carrying out ultrasonic emulsification to obtain an emulsion; and (3) stirring the emulsion obtained in the step (2), carrying out centrifuging, and washing a precipitate with water to obtain the multifunctional ultrasonic contrast agent. The contrast agent disclosed by the invention is a multifunctional contrast agent integrating near-infrared fluorescence/magnetic resonance/ultrasonic multi-mode development and photothermal therapy, and realizes photothermal therapy under the guidance of multiple image modes.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a multifunctional ultrasonic contrast agent and a preparation method thereof. Background technique [0002] The role of conventional ultrasound in imaging many organs in the human body is limited by the capabilities of conventional color and spectral Doppler imaging. Doppler imaging has limited ability to detect blood flow information from perfusion levels, especially low-velocity blood flow. However, in the contrast-enhanced ultrasound mode, the ultrasound contrast agent uses microbubbles to generate strong nonlinear harmonic echoes, which enhances the Doppler signal in the blood, and can display tissue microcirculation and tissue perfusion. The contrast agent widely used clinically is a blood pool contrast agent with a diameter of 1-8um. With the continuous development of nanomedicine, nanotechnology can be applied to biomedical imaging, monitoring, diagnosis ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22A61K49/18A61K49/12A61K49/00A61K41/00A61P35/00
CPCA61K49/225A61K49/1857A61K49/12A61K49/0032A61K49/0093A61K49/0002A61K41/0052A61P35/00
Inventor 牛诚诚汪龙陈思洁
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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