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Application of long-chain non-coding RNA MIR210HG in diagnosis and treatment of preeclampsia

A preeclampsia and drug treatment technology, applied in the fields of biomedicine and molecular biology, can solve the problems of deep placental formation defects, failure of uterine spiral artery remodeling, excessive proliferation of trophoblasts, etc.

Pending Publication Date: 2020-07-28
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that lnc-H19 may regulate gene expression through post-transcriptional mechanisms and imprinting regulation, leading to excessive proliferation of trophoblasts in early-onset severe PE by down-regulating miR-675 and interfering with nodal signaling
lnc-MEG3 participates in the apoptosis of trophoblast cells through NF-κB and Bax / Bcl2 signaling pathways, thereby promoting the failure of uterine spiral artery remodeling and resulting in defects in deep placental formation
However, the inventors found that there are still few reports on lncRNAs related to preeclampsia

Method used

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  • Application of long-chain non-coding RNA MIR210HG in diagnosis and treatment of preeclampsia
  • Application of long-chain non-coding RNA MIR210HG in diagnosis and treatment of preeclampsia
  • Application of long-chain non-coding RNA MIR210HG in diagnosis and treatment of preeclampsia

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Experimental procedure:

[0063] 1. Research object

[0064] From January 2006 to June 2019, a total of 200 cases of medical records were collected by the prenatal diagnosis laboratory, divided into PE (PE) group and normal control group, and the case data and placental tissue samples were collected.

[0065] 2. Collection of specimens

[0066] Immediately after the placenta was delivered by cesarean section, two pieces of placenta with a size of about 1cm×1cm×1cm and a little decidua tissue were taken from the center of the mother’s surface of the placenta in an aseptic state. The specimens were placed in a sterile Eppendorf tube and placed in liquid nitrogen for 15 minutes. Save until RNA extraction. Real-time PCR was used to detect the MIR210HG mRNA level in the placenta tissue of each group.

[0067] Total RNA was isolated with Trizol reagent according to the instructions of the reagents. Reverse transcription reaction using TaKaRa Prime

[0068] Script kit (Ta...

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Abstract

The invention provides an application of lncRNA MIR210HG in diagnosis and treatment of preeclampsia, and belongs to the technical field of biological medicines and molecular biology. Researches find that the up-regulation of the MIR210HG expression in the placenta tissue of a PE pregnant woman is proved for the first time. The MIR210HG expression is knocked down in the placenta trophoblast HT8 / SVneo of the PE pregnant woman, and trophoblast cells show an increase in cell proliferation and migration. The invention provides a new PE pathogenic mechanism, which is expected to provide a new targetand theoretical basis for diagnosis and treatment of PE, thus having good practical application value.

Description

technical field [0001] The invention belongs to the technical fields of biomedicine and molecular biology, and specifically relates to the application of long-chain non-coding RNA MIR210HG in the diagnosis and treatment of preeclampsia. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Pre-eclampsia (Pre-eclampsia, PE) is a hypertensive disorder that occurs during pregnancy, affecting about 5-8% of pregnant women, and PE / eclampsia is related to 10-15% of maternal direct deaths. PE is associated with eclampsia and HELLP syndrome in the mother, as well as preterm birth, intrauterine growth restriction, and perinatal death in the fetus. PE has adverse effects on maternal and ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883A61K45/00A61P15/00
CPCA61K45/00A61P15/00C12Q1/6883C12Q2600/112C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 刘世国张璐刘文淼
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV