Bioactive substance freeze-dried solid mask and preparation method thereof

A bioactive, freeze-drying technology, applied in the field of skin care products, can solve the problems of fast expiration of cytokines, short storage time at room temperature, and low actual effect, and achieve the effects of softening stratum corneum protein, reducing wrinkle growth, and relieving stress.

Active Publication Date: 2020-08-18
和携科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition to traditional beauty ingredients, beauty products added with biological factors have appeared on the market in recent years, mainly including epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), acidic fibroblast growth factor (aFGF) ), keratinocyte growth factor (KGF); the use of the activity of biological factors themselves can promote the removal of aging skin cells and the repair of cells, thereby improving the skin condition; however, although biological factors have good activity, their storage time at room temperature Short, cytokines fail quickly, so the actual effect is not high; therefore, the research and development of more efficient skin care products that can promote skin beauty, moisturize and repair damaged skin is also a research hotspot in the field of skin care products

Method used

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  • Bioactive substance freeze-dried solid mask and preparation method thereof
  • Bioactive substance freeze-dried solid mask and preparation method thereof
  • Bioactive substance freeze-dried solid mask and preparation method thereof

Examples

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preparation example Construction

[0040] Preparation example of adipose-derived mesenchymal stem cell lysate

[0041] Preparation example 1 of adipose-derived mesenchymal stem cell lysate: A, solution preparation: add serum substitute to DMEM / F12 basal medium to make a medium containing 4% serum substitute, labeled as solution C; Add 20 mL of 0.25% trypsin to the saline, mix well, and mark it as solution D.

[0042] B. Cell recovery:

[0043] b1. Add 10mL of DMEM / F12 basal medium to a 15mL centrifuge tube, preheat to 37°C, and let stand for later use;

[0044] b2. After taking out the cryopreservation tube containing human adipose tissue-derived seed mesenchymal stem cells from the liquid nitrogen tank, quickly put it into a 37°C water bath, place the cryopreservation tube upright with the tube cap up and above the water surface, Shake the cryovial quickly to melt the cryopreservation solution in the cryovial quickly;

[0045] b3. After thawing the cryopreservation solution (seed mesenchymal stem cells) of ...

preparation example 2

[0098] Preparation example 2 of adipose-derived mesenchymal stem cell lysate: The difference between this preparation example and the preparation example 1 of adipose-derived mesenchymal stem cell lysate is that step d18 is not included, and the cell lysate is directly vacuumed after obtaining the cell lysate at d17 Freeze-dry to obtain adipose-derived mesenchymal stem cell lysate.

preparation example 3

[0099] Preparation example 3 of adipose-derived mesenchymal stem cell lysate: the difference between this preparation example and the preparation example 1 of adipose-derived mesenchymal stem cell lysate is that in step d18, β-cyclodextrin and trehalose are not added to the cell lysate.

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Abstract

The invention relates to the field of skin care products, in particular to a bioactive substance freeze-dried solid facial mask and a preparation method thereof. The bioactive substance freeze-dried solid facial mask is prepared from a facial mask cloth soaked with a facial mask liquid through low-temperature drying, the mask liquid comprises a phase A, a phase B and a phase C, the phase A is prepared from water, glycerol, propylene glycol, plant extract, sodium hyaluronate, tranexamic acid, raspberry ketone glucoside, sclerotium gum, erythritol, allantoin, a thickening agent and p-hydroxyacetophenone, the phase B is prepared from olive fruit oil, grape seed extract, jojoba seed extract, sweet orange essential oil, vitamin E, dioctyl carbonate and hydrogenated lecithin, and the phase C isan adipose tissue-derived stromal cell lysate. The bioactive substance solid mask disclosed by the invention has the effects of whitening and moisturizing the skin, improving the glossiness of the skin and repairing acne marks and scar skin.

Description

technical field [0001] The invention relates to the technical field of skin care products, more specifically, it relates to a freeze-dried solid mask of biological active substances and a preparation method thereof. Background technique [0002] Mask is a category of skin care products and a carrier of beauty care products. It is applied on the face for beauty functions such as moisturizing, whitening, anti-aging, and oil balance. The forms of facial masks mainly include mud cream type, tear-off type, jelly type and wet tissue type; common mud cream-type masks include seaweed masks, mineral mud masks, etc. The gel-type mask is mainly a sleeping mask, and the wet tissue-type mask is generally a mask paper soaked with beauty essence in a single package. In recent years, as people have diversified needs for skin care, new types of facial masks that are different from traditional facial masks have emerged, among which freeze-dried facial masks are a new type of facial masks. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/02A61K8/34A61K8/44A61K8/49A61K8/60A61K8/67A61K8/73A61K8/92A61K8/9789A61K8/99A61Q19/00A61Q19/02
CPCA61K8/0212A61K8/44A61K8/345A61K8/735A61K8/602A61K8/73A61K8/4946A61K8/9789A61K8/922A61K8/678A61K8/99A61Q19/02A61Q19/00A61K2800/5922
Inventor 杨桂花赵进军赵宇飞
Owner 和携科技有限公司
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