Novel high-performance culture bottle suitable for suspension and adherent cell mixed culture

Abstract

The invention discloses a novel high-performance culture bottle suitable for suspension and adherent cell mixed culture. The culture bottle comprises a culture bottle body, wherein the culture bottlebody comprises an upper culture bottle shell and an adherent cell culture plate; the upper culture bottle shell covers the top of the adherent cell culture plate; an upper breathable film is arrangedin the center of the top wall of the upper culture bottle shell; a bottle opening is formed in one side of the upper culture bottle shell; a bottle cap is arranged on the bottle opening; inner fixingsupport columns are arranged at four corners of the top end of the adherent cell culture plate; the adherent cell culture plate is provided with a plurality of bottom breathable grooves formed in thebottle opening direction, and a bottom breathable film is arranged at the bottom of the adherent cell culture plate. The culture operation process of mixed cells is greatly simplified, and the problemof complexity of a traditional multi-culture-bottle is solved; by adopting a double-sided breathable film body structure and a bottom adherence plate vent groove structure, the mass transfer efficiency in the cell culture process is greatly improved, and the culture efficiency is effectively improved.

Description

technical field [0001] The invention relates to the technical field of cell culture bottles, in particular to a novel high-performance culture bottle suitable for mixed culture of suspension and adherent cells. Background technique [0002] Various types of cell therapy drugs have entered a period of rapid development. Some types of cell therapy methods require a variety of cells to be used together to produce target efficacy, such as the widely used DC-CIK treatment method, CIK (suspension cells) are used as actual killing and immune efficacy cells, DC cells (adherent cells) Used as antigen-presenting cells to guide CIK cells to produce target efficacy. Suspension cells have a large culture density and total volume, requiring higher flux mass transfer (gas) exchange in the culture environment, and adherent cells require a smooth adherent surface for stable growth. In the traditional process, different culture flasks are usually used for suspension cells and adherent cells...

AI Technical Summary

Problems solved by technology

In the traditional process, different culture flasks are usually used for suspension cells and adherent cells to meet different culture needs. The operation in the process is complicated and cumbersome, and at the same time, it cannot effectively prod

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