Method for establishing PK-PD synchronous model of tilmicosin to lawsonia intracellularis of pigs

Abstract

The invention discloses a method for establishing a PK-PD synchronous model of tilmicosin to Lawsonia intracellularis of pigs. The method comprises the following steps: drawing a growth curve of Lawsonia intracellularis in IPEC-J2 cells; determining intracellular and extracellular minimum inhibitory concentrations, anti-mutation concentrations and antibacterial post-effects of tilmicosin on lawsonia intracellular and extracellular of pigs, and drawing in-vitro and semi-in-vivo intracellular sterilization curves; selecting proper PK-PD parameters according to a measured intracellular sterilization curve in a lawsonia intracellularis half body, fitting data, establishing a PK-PD synchronous model of tilmicosin to lawsonia intracellularis, and formulating a reasonable dosing scheme. Accordingto the invention, an administration scheme of tilmicosin to Lawsonia intracellularis can be formulated, stable medication data support is provided for scientific culture, and clinical medication is scientifically guided; the drug resistance of the lawsonia intracellularis to the tilmicosin is effectively relieved, the effectiveness of the tilmicosin is protected and maintained, and a guide is provided for researching the PK-PD synchronous model of the antibacterial drug to the intracellular bacteria.

Description

technical field [0001] The invention relates to the technical field of PK-PD synchronization models, in particular to a method for establishing a PK-PD synchronization model of tilmicosin on Lawsonia intracellularis. Background technique [0002] Lawsonia intracellularis (L.intracellularis) belongs to the genus Desulfovibrio, a Gram-negative bacterium that is curved, comma-shaped or S-shaped, with a size of 1.25-1.75 μm×0.25-0.43 μm. Obligately parasitizes intestinal cells in animals and causes porcine proliferative enteritis (PPE) characterized by adenomatous proliferation of immature enterocytes in the ileum and colonic crypts. Although the mortality rate of PPE is not high, the disease can cause clinical symptoms such as diarrhea and slow growth in sick pigs, seriously reducing the feed utilization rate of animals, and causing huge economic losses. [0003] With the increasing resistance of porcine proliferative enteritis to antibiotics, more and more drugs can no longer...

AI Technical Summary

Problems solved by technology

Although the mortality rate of PPE is not high, the disease can cause clinical symptoms such as diarrhea and slow growth in sick pigs, seriously reducing the feed utilization rate of animals, and causing huge economic losses

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