Application of branched aminotransferase 1 and/or branched aminotransferase 2

A technology of branched-chain aminotransferase, which is applied in the application field of branched-chain aminotransferase 1 and/or branched-chain aminotransferase 2, and can solve problems such as unreported correlation studies

Active Publication Date: 2020-08-21
CHINA PHARM UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the correlation between BCAT1 and BCAT2 in acute myocardial infarction

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of branched aminotransferase 1 and/or branched aminotransferase 2
  • Application of branched aminotransferase 1 and/or branched aminotransferase 2
  • Application of branched aminotransferase 1 and/or branched aminotransferase 2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Acute myocardial infarction significantly reduces the expression of BCAT1 and BCAT2

[0050] The mouse OGD-induced myocardial injury cell model and acute myocardial infarction model were constructed, and the expression differences of BCAT1 and BCAT2 in the two animal models and the control group were analyzed by Western blot.

[0051] Such as figure 1 As shown, the expressions of BCAT1 and BCAT2 were significantly reduced in the myocardial injury cell model induced by OGD, and the expressions of BCAT1 and BCAT2 were also significantly reduced in acute myocardial infarction model mice ( Figure 2-3 ).

Embodiment 2

[0052] Example 2 Overexpression of BCAT1 and BCAT2 can reduce myocardial damage caused by acute myocardial infarction

[0053] In order to explore the role of BCAT1 and BCAT2 in acute myocardial infarction, myocardial injection of adeno-associated virus carrying BCAT1 and BCAT2 genes was used to overexpress BCAT1 and BCAT2. After 4 weeks of transfection, 50% transfection rate was observed by immunohistochemical method, and then sham operation and acute myocardial infarction model were performed. The results showed that overexpression of BCAT1 and BCAT2 significantly reduced infarct size ( Figure 4 ), decreased the activity of CK and LDH ( Figure 5 ), and improved the histopathological characteristics of transfected mice ( Image 6 ). However, overexpression of BCAT1 and BCAT2 had no significant effect on sham-operated mice. In addition, overexpression of BCAT1 and BCAT2 had no effect on the expression of OXE-R. After acute myocardial infarction modeling, over-expression...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses application of branched aminotransferase 1 and / or branched aminotransferase 2. The invention for the first time finds that expression of branched aminotransferase 1 (BCAT1) andbranched aminotransferase 2 (BCAT2) in the cardiac tissue of an acute myocardial ischemia model mouse is abnormally reduced, and the phenomenon of abnormal reduction of expression also occurs in an oxygen-glucose deprivation induced myocardial injury cell model. Cardiac in-situ BCAT1 and BCAT2 protein overexpression is carried out on the mouse respectively, thus finding that myocardial ischemia injury can be effectively relieved. Therefore, the BCAT1 and the BCAT2 are proved to be able to serve as novel treatment targets for screening drugs for preventing, relieving and / or treating myocardialischemia injury.

Description

technical field [0001] The invention belongs to the field of medicine and relates to the application of branched chain aminotransferase 1 and / or branched chain aminotransferase 2. Background technique [0002] Acute myocardial infarction (AMI) is a very common disease worldwide with high mortality and morbidity, characterized by reduced oxygen and blood supply to the heart. At present, the diagnostic methods of myocardial infarction mainly include high-sensitivity troponin detection and electrocardiogram detection. High-sensitivity troponin detection has higher sensitivity but lower specificity for myocardial infarction. Therefore, ECG detection is still the main method for the diagnosis of myocardial infarction. The clinical treatment of acute myocardial infarction includes drug therapy, coronary artery stent implantation surgery, and coronary artery bypass surgery, combined with cardiac rehabilitation. The current commonly used therapeutic drugs mainly include aspirin, c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573A61K45/00A61P9/10
CPCG01N33/573A61K45/00A61P9/10G01N2800/324G01N2333/91188G01N2800/52
Inventor 李芳余伯阳赖琼寇俊萍袁光英
Owner CHINA PHARM UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap