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A kind of ester catechin synthase, encoding gene and application thereof

A technology of ester catechin and synthetase, which is applied in the directions of application, genetic engineering, plant genetic improvement, etc., can solve the problem that the formation mechanism of catechin gallate is not determined.

Active Publication Date: 2022-04-08
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it is known that there are generally two types of enzymes that catalyze the esterification of such organic acids with flavonoids such as flavantriols: BAHD and SCPL family enzymes, because their respective families include many members, no tea tree species have been identified so far. The formation mechanism of catechin gallate in

Method used

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  • A kind of ester catechin synthase, encoding gene and application thereof
  • A kind of ester catechin synthase, encoding gene and application thereof
  • A kind of ester catechin synthase, encoding gene and application thereof

Examples

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Embodiment

[0034] Example Cloning, expression and detection of enzymatic properties of serine carboxypeptidase acyltransferase gene

[0035]1. Cloning of CsSCPL11-IA, CsSCPL13-IA and CsSCPL14-IA genes

[0036] The primer sequences of the three genes are shown below.

[0037] >CsSCPL11-IA-F

[0038] ATGTTTCCACCAAAGTCATA, SEQ ID NO. 7;

[0039] >CsSCPL11-IA-R

[0040] CTAAATAGGATAGTAATGAA, SEQ ID NO. 8;

[0041] >CsSCPL13-IA-F

[0042] ATGGTGCAAGTAGAAGCCAT, SEQ ID NO. 9;

[0043] >CsSCPL13-IA-R

[0044] AACAGGATTATAATTAATCC, SEQ ID NO. 10;

[0045] >CsSCPL14-IA-F

[0046] ATGGTGCAAGTAGAAGCCAT, SEQ ID NO. 11;

[0047] >CsSCPL14-IA-R

[0048] CTAGGAGTGGTGAGTTAGGT, SEQ ID NO. 12;

[0049] According to the instructions of TIANGEN Plant Total RNA Extraction Kit and M-MLV cDNA Synthesis Kit, the total RNA of Shucha early young leaves was extracted and reverse transcribed into cDNA.

[0050] Using reverse-transcribed cDNA as a template, primers and TAKARA ExTaq polymerase were used fo...

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Abstract

The invention belongs to the technical field of bioengineering, and in particular relates to an ester-type catechin synthase, a coding gene and an application thereof. The present invention obtains recombinant protein by constructing recombinant engineering bacterium, by means of enzyme reaction method, utilizes the recombinant protein of genetic engineering bacterium as catalytic enzyme, verifies the serine carboxypeptidase class acyl transferase CsSCPL11-IA, CsSCPL13-IA and CsSCPL13-IA in the tea tree Gene of CsSCPL14‑IA. The invention firstly describes three acetyltransferase genes that can catalyze the synthesis of ester-type catechins, and their application value in the breeding of fine tea varieties and tea processing.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to an ester-type catechin synthase, a coding gene and an application thereof. Background technique [0002] Tea polyphenols is a natural polyphenol compound extracted from tea, accounting for about 25% of the dry weight of tea. It is a general term for more than 30 polyphenol compounds in tea. one of the main medicinal ingredients. Catechins are one of the main components of tea polyphenols, accounting for 50% to 70% of its total weight. The catechins in tea contain multiple components, which are mainly divided into ester type and non-ester type catechin. Non-ester catechins include catechin (C, (+)-catechin), epicatechin (EC, (-)-epicatechin), gallate catechin (GC, (+)-gallocatechin, and epicatechin Epicatechin (EGC, (-)-epigallocatechin). Ester catechins include epicatechin gallate (ECG, (-)-epicatechin-3-gallate) and epigallocatechin-3-gallate Ester (EGCG, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/10C12N15/54C12P17/06C12Q1/6895A23F3/06A01G17/00
CPCC12N9/1029C12P17/06C12Q1/6895A23F3/06A01G17/005C12Q2600/13
Inventor 赵剑左飞李鹏辉曾祥圣
Owner ANHUI AGRICULTURAL UNIVERSITY
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