Method for improving drought resistance of dodonaea viscosa at seedling stage through local arbuscular mycorrhiza fungi
A technology of arbuscular mycorrhizal fungi and drought resistance, which is applied in the directions of seed and rhizome treatment, botanical equipment and methods, and application, etc., to achieve good economic and ecological benefits, promote biomass accumulation, and have remarkable effects on growth promotion.
Inactive Publication Date: 2020-08-28
MIANYANG TEACHERS COLLEGE
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[0007] In order to solve the defect that the prior art can not well solve the drought resistance of the seedling stage of the mulberry seedling, the invention discloses a method for improving the drought resistance of the seedling stage of the mulberry seedling by using native arbuscular mycorrhizal fungi, and the purpose of realization is directly from The arbuscular mycorrhizal fungal community was isolated from the rhizosphere soil of Mulberry in dry-hot valley and inoculated on the local soil to promote the ecological application of arbuscular mycorrhizal fungi in the local soil environment
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Embodiment 1
[0025] Embodiment one: a kind of method that utilizes local arbuscular mycorrhizal fungus to improve the drought resistance of Fructus mulberry seedling stage provided by the present invention comprises: (1) soil collection: A. rhizosphere soil collection: in dry hot valley area, Yuanmou County, Yunnan Province Before the onset of the rainy season in May, the rhizosphere soil samples were collected by the shaking soil method, that is, the soil that remained at the root after shaking the plant gently was the rhizosphere soil, and the rhizosphere soil was collected in a ziplock bag with a brush. They were transported back to the laboratory in an ice box and stored in a refrigerator at 4°C for later use.
[0026] B. Pot soil collection: Select the area where the mulberry fruit grows well in the field, and use the method of multi-point mixed sampling to collect the dry red soil surface soil (0-20cm), remove the litter and large gravel from the collected soil, and transport it back ...
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The invention discloses a method for improving drought resistance of dodonaea viscosa at a seedling stage through local arbuscular mycorrhiza fungi. The method comprises the steps of (1) collecting soil: collecting rhizosphere soil and collecting pot culture soil; (2) preparing pot culture substrates: performing sterilization and uniform mixing on the pot culture soil in the step (1) to obtain a culture medium for growth of dodonaea viscosa; (3) separating local arbuscular mycorrhiza fungi: separating local arbuscular mycorrhiza fungal spores from the rhizosphere soil sample collected in the step (1); (4) performing fungus inoculation: inoculating the culture medium with the separated fungal spores; (5) performing seed treatment: breaking down dormancy of the collected seeds, sowing the treated seeds in the culture medium prepared in the step (4), after sowing, covering sterilization substrates on the surface, and enabling seeds not to be exposed; and (6) performing seedling stage management and observation. The method disclosed by the invention has the advantages that mutual adaptation relationship between plants, arbuscular mycorrhiza fungus communities and soil environment is fully utilized, and the method has important reference value on promotion of growth and development of the dodonaea viscosa and other local plants under the drought condition.
Description
technical field [0001] The invention belongs to the technical field of plant cultivation, and specifically relates to a method for improving the drought resistance of mulberries by using native arbuscular mycorrhizal fungi. Background technique [0002] Dodonaea viscosa Jacq. is one of the most widely distributed species in the world. The only Sangzi in my country is distributed in the southwest, south to southeast, also known as Poliu and Mingyouzi. It is the construction species of the vegetation and shrubs in the dry and hot valleys of the Southwest. Sangzi has important medicinal value and pesticide value. It has the characteristics of drought resistance, strong germination, well-developed root system, and cluster growth. However, the filtering effect of drought on the seedling stage of S. mulberry is very significant, and the management and protection at the seedling stage should be especially strengthened in the process of vegetation restoration. [0003] Arbuscular ...
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IPC IPC(8): A01G17/00A01G18/10A01C1/00
CPCA01C1/00A01G17/005A01G18/10
Inventor 王雪梅闫帮国赵广史亮涛刘刚才
Owner MIANYANG TEACHERS COLLEGE