Fluorescent probe for detecting butyrylcholinesterase activity as well as synthesis method and application of fluorescent probe

Abstract

The invention discloses a fluorescent probe for detecting butyrylcholinesterase activity as well as a synthesis method and application of the fluorescent probe. The invention discloses a novel butyrylcholinesterase sensing strategy established on the basis of background-signal-free detection, and under the action of butyrylcholinesterase, fluorescence signals develop from nothing so that the concentration level of butyrylcholinesterase is detected. According to the fluorescent probe, a targeting site of butyrylcholinesterase is introduced into a non-emission skeleton to generate a probe P1, sothat the inherent fluorescent background of the detection probe is avoided, and the detection sensitivity is greatly improved. After butyrylcholinesterase is added, cyclopropylbutyric acid can be removed, and then spontaneous in-situ cyclization is performed to generate a fluorescent product PF to serve as an indicator for reflecting the activity of BChE. The fluorescent probe has greatly improved detection sensitivity, but also has good selectivity and biocompatibility, and has great potential in the field of clinical diagnosis.

Description

technical field [0001] The invention belongs to the technical field of detection of butyrylcholinesterase activity, and relates to a method strategy for detecting the activity level of butyrylcholinesterase in cells, tissue sections and organisms. More specifically, it relates to a non-emission probe, its synthesis method and its use as an indicator by spontaneous cyclization to generate a fluorophore when detecting butyrylcholinesterase. Background technique [0002] Butyrylcholinesterase (BChE) is also known as pseudocholinesterase or cholinesterase II. Since BuChE is released into the blood immediately after synthesis in the liver, it can be used as a sensitive indicator for evaluating the synthetic function of liver cells. Determination of the concentration of BChE in serum is generally used as a liver function test. It has been reported that BChE is also closely related to complex neurodegenerative diseases such as Alzheimer's disease (AD). With the development of AD, ...

AI Technical Summary

Problems solved by technology

For the first three methods, it is difficult to avoid the problems of complex operation, low sensitivity and poor stability, which hinder their further application

However, although the DTNB assay has high sensitivity with thiobutyrylcholine as the substrate, it may be less accurate due to potential interference from the detection system

And in the hydroxylamine colorimetric method, acetylcholine is used as a reactive substrate, which is not conducive to the determination of BChE, resulting in an inevitable decrease in detection sensitivity

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