Application method of two insecticides

An application method, insecticide technology, applied in the field of application of phoxim and lambda-cyhalothrin, can solve the problem of not providing guidance, etc.

Active Publication Date: 2020-08-28
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the compound insecticide of phoxim and lambda-cyhalothrin is still used on a large scale. Although the toxic effect of single pollution of phoxim and lambda-cyhalothrin has been studied, so far there is no Regarding the report on the joint chronic toxicity effect of phoxim and lambda-cyhalothrin on earthworms, it is not clear how to protect the soil ecosystem and reduce pollution when the two pesticides are applied to the same soil. Intensive research for proper application and no corresponding guidance on such application methods

Method used

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  • Application method of two insecticides
  • Application method of two insecticides
  • Application method of two insecticides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 phoxim is to earthworm toxicity test

[0036] Dissolve a certain amount of phoxim in acetone and mix it in 10g of quartz sand or directly mix it in 10g of quartz sand. After the acetone is completely volatilized, mix it with 490g of artificial soil. Add distilled water to adjust the soil humidity to the maximum water holding capacity. 40%. The above-mentioned phoxim-containing soil was put into an 800 mL beaker, and 10 adult earthworms domesticated for 7 days in the artificial soil without phoxim treatment were added to the beaker. After the earthworms entered the artificial soil, 5 g of oatmeal was evenly spread on the soil surface as food for the earthworms, and an appropriate amount of deionized water (5-6 mL) was sprayed to make the oatmeal moist. Seal the mouth of the glass bottle with a plastic film, then pierce the plastic film with a dissecting needle and place it in an incubator at 20±1°C, with a light-to-dark ratio of 16h:8h, and a humidity of 80...

Embodiment 2

[0037] Example 2 Beta-cyhalothrin Toxicity Test to Earthworms

[0038] Beta-cyhalothrin is the same as embodiment 1 for the test method of poisoning earthworm reproduction. Eight concentrations of lambda-cyhalothrin are set according to a certain level difference (respectively 0.01mg / kg, 0.03mg / kg, 0.09mg / kg, 0.27mg / kg, 0.81mg / kg, 2.43mg / kg, 7.29mg / kg and 21.87mg / kg), the set concentration range completely covers the industry recommended dosage of lambda-cyhalothrin (0.01-2mg / kg), and each concentration has 3 repetitions (each beaker is 1 repetition). 8 blank control groups without drug. Adopt DPS statistical analysis software (version number: V14.10), utilize probability value analysis method to carry out statistical analysis to earthworm reproductive toxicity data, obtain EC 50 and its 95% confidence limits.

Embodiment 3

[0039] Example 3 Toxicity test of phoxim and lambda-cyhalothrin combined exposure to earthworms

[0040] This example relates to the toxicity test of mixed pesticides of phoxim and lambda-cyhalothrin on earthworm reproduction using the methods of equal toxicity and equal concentration respectively. Equal toxicity design using a single pesticide on the EC of inhibiting the number of earthworm larvae 50 The value is one toxicity unit, and the mixed pesticides are set at 8 different concentrations at equal logarithmic intervals according to the mixing ratio of two single pesticide toxicity units ratio of 1:1 (0.075 mg / kg for phoxim and lambda-cyhalothrin, respectively). +0.01mg / kg, 0.22mg / kg+0.03mg / kg, 0.67mg / kg+0.09mg / kg, 2.02mg / kg+0.27mg / kg, 6.06mg / kg+0.81mg / kg, 18.18mg / kg +2.43mg / kg, 54.53mg / kg+7.29mg / kg, 163.58mg / kg+21.87mg / kg concentration mixed), the exposure test method is the same as that of Example 1. When the concentration is equal, two pesticides, phoxim and lambda-c...

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Abstract

According to the invention, evaluation of earthworm propagation toxicity by combined exposure of phoxim and lambda-cyhalothrin is carried out; by researching the application isolation period, it is proposed that the two insecticides need to be applied in the same soil at intervals, the application dosage of phoxim is 1-50 g (effective components)/mu, the application dosage of lambda-cyhalothrin is0.1-20 g (effective components)/mu, and the application interval is not shorter than 1 week. Preferably, when phoxim is firstly applied to the same soil and then lambda-cyhalothrin is applied, the application interval is 3 weeks; when the lambda-cyhalothrin is applied firstly and then the phoxim is applied, the application interval is 2 weeks. According to the invention, a mixture of phoxim and lambda-cyhalothrin should be cautiously used in agricultural production; when phoxim and lambda-cyhalothrin are applied to the same soil, the application interval must be strictly carried out, andtherefore, adverse effects on the ecological environment are reduced, references are provided for research of combined exposure of other different kinds of pollutants on earthworm chronic toxicity tests and application intervals, and important scientific bases are also provided for monitoring, early warning and remediation treatment of soil combined pollution.

Description

technical field [0001] The invention relates to the field of prevention and control of soil toxicity pollution, in particular to the application method of two pesticides, especially the application method of phoxim and lambda-cyhalothrin. Background technique [0002] In agricultural production, in order to control harmful organisms such as pests and weeds, it is often necessary to use multiple pesticides simultaneously or successively. In addition, in order to expand the control targets or improve the control effect on a single pest and delay the emergence of drug resistance, a large number of pesticide mixtures are also used. was put into use. There are often complex interactions between different pesticides, which affect each other's absorption, distribution, metabolic transformation and toxic effects, forming compound pollution effects, such as synergistic, additive and antagonistic effects. At present, many ecotoxicological effects cannot be explained by the mechanism ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50A01N57/14A01N53/00
CPCG01N33/5085A01N57/14A01N53/00
Inventor 王彦华李薪芳狄珊珊杨桂玲王强
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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