Radix pseudostellariae saponin and polysaccharide extract, radix pseudostellariae and lucid ganoderma wine and preparation method
A technology for polysaccharide extraction and ginsenosides, which is applied to the field of Radix Radix Ganoderma lucidum wine and preparation, Radix Radix Glossom saponins and polysaccharide extracts, can solve the problems of low extraction efficiency of effective components and waste of Radix Radix Ginseng resources, and achieves reduction of purification steps, antioxidant The effect of strong performance and improved utilization
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[0019] The present invention provides a kind of preparation method of heterophylla pseudoginsenoside and polysaccharide extract, comprising the following steps:
[0020] 1) Mix the ethanol aqueous solution with a volume percentage of 35-45% of Radix Pseudostellariae in accordance with a solid-liquid ratio of 1:(25-35) g / mL, and perform ultrasonic extraction for 35-45 minutes to obtain Pseudostellaria saponin extract and Pseudophylloides Radix Slag ;
[0021] 2) Mix the Radix Pseudostellaria residue obtained in step 1) with water according to the ratio of material to liquid of 1:(25-35) g / mL, boil and extract in water at 75-85°C for 110-120 minutes, and obtain the polysaccharide extract of Radix Pseudostellariae.
[0022] In the present invention, the participation volume percentage of Prince Edward is 35-45%, more preferably 40% ethanol aqueous solution according to the ratio of material to liquid is 1: (25-35) g / mL, more preferably 1:30 g / mL mixed, ultrasonic Extract for 35 ...
Embodiment 1
[0034] Time: July 10, 2017, Location: School of Food Science, Fujian Agriculture and Forestry University.
[0035] 1. Pass the pulverized Taizi ginseng through a 30-mesh sieve, then mix it with an aqueous ethanol solution with a volume percentage of 35% according to a solid-liquid ratio of 1:25g / mL, and use 100W power ultrasonic extraction for 35min at 55°C to obtain Taizi Ginsenoside extract and Pseudostellaria slag.
[0036] 2. Dry Pseudostellariae residue at 50°C, then mix the dried Pseudostellariae Pseudostellariae residue with water at a material-to-liquid ratio of 1:25g / mL, and extract by boiling at 75°C for 110 minutes to obtain a polysaccharide extract of Pseudostellariae heterophylla.
[0037] The yield of saponin in the first step of extracting saponin is 0.481%, and the yield of polysaccharide under this process condition is 0.318%; in the second step of extracting polysaccharide, the yield of polysaccharide is 5.96%.
Embodiment 2
[0039] 1. Pass the pulverized Taizi ginseng through a 40-mesh sieve, then mix it with ethanol aqueous solution with a volume percentage of 40% according to the ratio of material to liquid at 1:30g / mL, and use 150W power ultrasonic extraction for 40min at 60°C to obtain Taizi Ginsenoside extract and Pseudostellaria slag.
[0040] 2. Dry Pseudostellariae residues at 60°C, then mix the dried Pseudostellariae Pseudostellariae residues with water at a material-to-liquid ratio of 1:30g / mL, and extract by boiling at 80°C for 115 minutes to obtain a polysaccharide extract of Pseudostellariae Radix.
[0041] The yield of saponin in the first step of extracting saponin was 0.466%, and the yield of polysaccharide was 0.312% under the conditions of this process; in the second step of extracting polysaccharide, the yield of polysaccharide was 6.13%.
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