Cross-linked collagen sponge with sustained release of ibuprofen and metronidazole and preparation method of cross-linked collagen sponge
A technology of collagen sponge and metronidazole, which is applied in the field of oral medical materials and its preparation, can solve the problems of inability to quickly stop bleeding, sterilize and relieve pain
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Embodiment 1
[0030] A cross-linked collagen sponge for sustained release of ibuprofen and metronidazole, comprising the following raw materials: 300mm 3 Cross-linked collagen sponge, 3.9mg metronidazole and 27mg ibuprofen. Prepared by supercritical solution impregnation method containing 13μg / mm 3 Concentration of metronidazole and 90μg / mm 3 Concentrated ibuprofen cross-linked collagen sponge. The prepared collagen sponge was stuffed in the wound after extraction of impacted teeth, and the gingiva was sutured.
[0031] Specific steps are as follows:
[0032] (1) Take fresh beef tendon, remove impurities such as fascia and fat, wash and cut into pieces, degrease in a 35°C water bath for 3 hours, sterilize in a 25°C water bath, add pepsin, and the pH is 1.6, the powder-to-liquid ratio (g / ml) under the condition of 1:20, extract for 24 hours to obtain a crude collagen solution, after inactivating the enzyme, purify by high performance liquid chromatography to obtain a purified collagen sw...
Embodiment 2
[0038] A cross-linked collagen sponge for sustained release of ibuprofen and metronidazole, comprising the following raw materials: 600mm 3 Cross-linked collagen sponge, 10.8 mg metronidazole and 58.2 mg ibuprofen. Prepared by supercritical solution impregnation method containing 18μg / mm 3 Concentration of metronidazole and 97μg / mm 3 Concentrated ibuprofen cross-linked collagen sponge. The cross-linked collagen sponge was stuffed into the wound after extraction of impacted teeth, and the gingiva was sutured.
[0039] Others are with embodiment 1.
Embodiment 3
[0041] A cross-linked collagen sponge for sustained release of ibuprofen and metronidazole, including the following raw materials: 1200mm 3 Cross-linked collagen sponge, 27.6mg metronidazole and 124.8mg ibuprofen. Made by supercritical solution impregnation method containing 23μg / mm 3 Concentration of metronidazole and 104μg / mm 3 Concentrated ibuprofen cross-linked collagen sponge. The prepared collagen sponge was stuffed in the wound after extraction of impacted teeth, and the gingiva was sutured.
[0042] Others are with embodiment 1.
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