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Synergistic denitrification method of ferroautotrophic bacteria and heterotrophic bacteria to remove nitrate in sewage

A technology of autotrophic and heterotrophic bacteria, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problems of low efficiency of iron autotrophic denitrification and achieve high efficiency and stability Nitrate removal effect, cost reduction, effect of reducing the risk of secondary pollution

Active Publication Date: 2021-05-11
HOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for synergistic denitrification of ferroautotrophic bacteria and heterotrophic bacteria to remove nitrate in sewage, so as to solve the problem in the prior art that heterotrophic denitrification needs an additional carbon source when processing low carbon-to-nitrogen ratio sewage, and iron The problem of low efficiency of autotrophic denitrification

Method used

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  • Synergistic denitrification method of ferroautotrophic bacteria and heterotrophic bacteria to remove nitrate in sewage
  • Synergistic denitrification method of ferroautotrophic bacteria and heterotrophic bacteria to remove nitrate in sewage

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Embodiment 1

[0025] The method for synergistic denitrification of ferroautotrophic bacteria and heterotrophic bacteria to remove nitrate in sewage provided by the embodiments of the present invention includes:

[0026] Step 1: Select the activated sludge from the sewage treatment plant, cultivate ferroautotrophic sludge and heterotrophic sludge in the first medium and the second medium respectively, add 6.00g / L FeSO to the first medium 4 ·7H 2 O and 0.73g / L NaNO 3 ; In the second medium, add 4.53g / L of CH 3 COONa and 0.73g / L NaNO 3 During the cultivation process of ferroautotrophic sludge and heterotrophic sludge, the respective mediums were replaced every two days. The culture conditions were to flow argon gas into the first medium and the second medium respectively for 30 minutes, and then seal them to keep anaerobic Oxygen state, and continuous dark treatment for 30 days;

[0027] Step 2: Put 10g of ferrous sulfide ore particles into a 500mL anaerobic bottle reactor, the particle si...

Embodiment 2

[0029] The content of embodiment 2 is basically the same as that of embodiment 1, and the difference is: in step 2, in the sewage with low carbon-to-nitrogen ratio, NO 3 - The concentration of -N was 20 mg / L.

Embodiment 3

[0031] The content of embodiment 3 is basically the same as that of embodiment 1, and the difference is: in step 2, in the sewage with low carbon-to-nitrogen ratio, NO 3 - The concentration of -N was 80 mg / L.

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Abstract

The invention discloses a method for synergistic denitrification of ferroautotrophic bacteria and heterotrophic bacteria to remove nitrate in sewage. Sludge was raised, and the culture conditions were to maintain anaerobic state, and continuously avoid light treatment; in the first medium, add ferrous ions and nitrate; in the second medium, add organic carbon source and nitrate; add ferrous sulfide Put the ore particles into the reactor, then inoculate the ferroautotrophic sludge and heterotrophic sludge into the reactor, and then inject sewage with low carbon-to-nitrogen ratio into the reactor to maintain anaerobic state and continue dark treatment. The hydraulic retention time is more than 72h; in low carbon nitrogen ratio sewage: C / N mass ratio is 0~1, NO 3 ‑ The concentration of ‑N is 20-80 mg / L; the present invention can efficiently remove nitrate in low-carbon-nitrogen-ratio sewage without additional carbon source through the synergistic denitrification effect of ferroautotrophic bacteria and heterotrophic bacteria.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, in particular to a method for synergistic denitrification of ferroautotrophic bacteria and heterotrophic bacteria to remove nitrate in sewage. Background technique [0002] Excessive discharge of nitrate in human production and living activities is one of the important reasons for water eutrophication and deterioration of water environment quality, and poses a threat to human health. The main technology currently applied to the removal of nitrate in sewage is biological denitrification, that is, the use of denitrifying bacteria to gradually reduce nitrate to nitrogen in an anaerobic environment and remove it from sewage. Biological denitrification includes heterotrophic denitrification and autotrophic denitrification, and the dominant microorganisms are heterotrophic bacteria and autotrophic bacteria, respectively. [0003] For sewage with a low carbon-to-nitrogen ratio, due to the lack...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C02F3/28C02F101/16
CPCC02F3/28C02F3/34C02F3/346C02F2101/163
Inventor 赵联芳王莉谭陈薛柳莹
Owner HOHAI UNIV
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