Devices for sample analysis using epitachophoresis
A sample analysis and epitope technology, applied in epitope electrophoresis (epitachophoresis) devices, sample analysis field, can solve problems such as difficulty in biological sample analysis
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[0336] CLAIMS 1. A device for sample analysis, wherein said device comprises or is in contact with an arrangement of one or more electrodes sufficient to perform epitope electrophoresis.
[0337] 2. The device of claim 1, wherein the device comprises a circular, spherical or polygonal geometric shape.
[0338] 3. The device of claim 2, wherein the epitope electrophoretic region of the device moves from the edge of the polygon or circle towards the center of the polygon or circle during use of the device in analyzing the sample.
[0339] 4. The device of claim 3, wherein the polygon is selected from the group consisting of triangles, quadrilaterals, pentagons, hexagons, heptagons, octagons, nonagons, decagons.
[0340] 5. The apparatus of claim 3, wherein the polygon has 3, 4, 5, 6, 7, 8, 9, 10-20, 20-50, or 50-100 or more sides.
[0341] 6. The device of any one of the preceding claims, wherein the arrangement of electrodes comprises a two-dimensional arrangement of one or mo...
example 1
[0472] Example 1: Device for Epitope Electrophoresis
[0473] For example, if Figure 1-Figure 4 Devices for epitope electrophoresis, as depicted in , typically use concentric or polygonal disk architectures. Glass or ceramics are used in the manufacture of the system ( which is, concentric or polygonal disks), as these materials lead to improved heat transfer properties, which is beneficial during device operation. For example, overheating (or boiling) of the focusing material is generally prevented due to the good heat transfer capabilities of the flat channels of the epitope electrophoretic device compared to narrow channels. Current / Voltage programming is also available to adjust the Joule heating of the device. Plastic materials are also used in device fabrication. Generally, the device is manufactured with a size that accommodates a desired sample volume, such as a sample volume in the milliliter range, for example up to 15 mL.
[0474] refer to Figure 1-Figure...
example 2
[0479] Example 2: Operation of an epitope electrophoresis device
[0480] Epitope electrophoresis devices such as Figure 1-Figure 4 those designs shown) operate in a two electrolyte cell arrangement, or in a three electrolyte cell arrangement with a lead electrolyte followed by mixing the sample with the stop electrolyte, or where the sample is first mixed with the lead electrolyte, This is followed by a terminating electrolyte such as Figure 5 presented in . In such an arrangement, the sample can be mixed with any conductive solution. Alternatively, the terminating electrolyte region can be eliminated when the sample contains suitable terminating ions. refer to Figure 2A-Figure 2B , after filling the terminating electrolyte cell (2) with a mixture of sample and a suitable terminating electrolyte and switching on the power supply (6), ions begin to move toward the center electrode (5) and between the leading and terminating electrolyte (7) A region is formed at the b...
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Abstract
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Application Information
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