Collagenase inhibitor composition, anti-aging moisture dew and preparation method of anti-aging moisture dew
A technology of inhibitor and collagenase, which is applied in the field of daily chemicals, can solve the problems of high molecular weight of hydrolyzed collagen and difficulty reaching the dermis, and achieve the effect of improving stability
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Embodiment 1-5
[0072] The wolfberry extract was diluted with deionized water to obtain 5 groups of test solutions with different concentrations (final concentrations). When performing the test for inhibiting collagenase activity, the mass concentration of the wolfberry extract is shown in Table 1 below.
[0073] Inhibition of Collagenase Activity Test
[0074] Folin's phenol reagent can be reduced by phenolic compounds under alkaline conditions to turn blue (a mixture of molybdenum blue and tungsten blue). The protein and its hydrolyzate exhibit the above reaction, so this principle can be used to measure protease activity. Usually, casein is used as a substrate to react with enzyme solution under certain pH and temperature conditions, and after a period of time, the enzymatic reaction is terminated with trichloroacetic acid, and the casein precipitate is removed by centrifugation or filtration, and the supernatant is taken. with NaOH and Na 2 CO 3 After alkalization, add Folin's phenol ...
Embodiment 6-10
[0092] Lycium barbarum extract was dissolved in 5 volumes of deionized water corresponding to Comparative Example 1 to obtain 5 groups of test solutions with different concentrations. When carrying out the inhibition mechanism determination of inhibiting collagenase activity, the mass concentration of Lycium barbarum extract is shown in Table 2 below.
[0093] Determination of the Inhibitory Mechanism of Lycium Barbarum Extract Inhibiting Collagenase Activity
[0094] Add the test solution of 0.25mL comparative example 1 and embodiment 6-10 in 36 test tubes respectively, and add 0.25mL, 0.2mL, 0.15mL, 0.1mL, 0.05mL, 0.025mL collagenase (32U / mL), and Add DPBS buffer solution with a pH of 7.4 to the final volume of 0.5 mL, then add 0.5 mL (1.0%) of the substrate casein solution and mix immediately, keep warm in a water bath at 37°C for 10 min, add 1 mL of trichloroacetic acid solution (6.5%) and mix. After standing for 10 minutes, centrifuge at 10,000 rpm for 5 minutes to obtai...
Embodiment 11-15
[0110] Lycium barbarum extract was dissolved in 5 volumes of deionized water corresponding to Comparative Example 1 to obtain 5 groups of test solutions with different concentrations. When measuring the inhibition mechanism of collagenase activity, the mass concentration of Lycium barbarum extract is shown in Table 3 below.
[0111] Determination of the Inhibition Types of Lycium Barbarum Extracts on Collagenase
[0112] Add 0.3mL, 0.2mL, 0.1mL, 0.05mL of substrate casein solution (1.0%) to 24 test tubes respectively, and add Tris·HCl buffer solution with pH 7.4 to a final volume of 0.5mL, then add 0.25 mL of the test solution of Comparative Example 2 and Examples 11-15, then add collagenase (32U / mL) and mix immediately, after 37 ° C water bath insulation for 10 min, then add 1 mL of trichloroacetic acid solution (6.5%) and mix, statically Centrifuge at 10,000 rpm for 5 minutes after leaving for 10 minutes to obtain the supernatant m of the control group.
[0113] Add 0.3mL,...
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