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Collagenase inhibitor composition, anti-aging moisture dew and preparation method of anti-aging moisture dew

A technology of inhibitor and collagenase, which is applied in the field of daily chemicals, can solve the problems of high molecular weight of hydrolyzed collagen and difficulty reaching the dermis, and achieve the effect of improving stability

Pending Publication Date: 2020-09-15
GUANGZHOU KENENG COSMETICS RES CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the existing technology, the molecular weight of hydrolyzed collagen is too large, and it is difficult to penetrate the skin barrier of the human body to reach the dermis; hyaluronic acid cannot essentially slow down the loss of collagen; peptides and retinol have certain irritation and safety risks to the skin etc., the present invention firstly provides a collagenase inhibitor composition for inhibiting collagenase activity

Method used

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  • Collagenase inhibitor composition, anti-aging moisture dew and preparation method of anti-aging moisture dew
  • Collagenase inhibitor composition, anti-aging moisture dew and preparation method of anti-aging moisture dew
  • Collagenase inhibitor composition, anti-aging moisture dew and preparation method of anti-aging moisture dew

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0072] The wolfberry extract was diluted with deionized water to obtain 5 groups of test solutions with different concentrations (final concentrations). When performing the test for inhibiting collagenase activity, the mass concentration of the wolfberry extract is shown in Table 1 below.

[0073] Inhibition of Collagenase Activity Test

[0074] Folin's phenol reagent can be reduced by phenolic compounds under alkaline conditions to turn blue (a mixture of molybdenum blue and tungsten blue). The protein and its hydrolyzate exhibit the above reaction, so this principle can be used to measure protease activity. Usually, casein is used as a substrate to react with enzyme solution under certain pH and temperature conditions, and after a period of time, the enzymatic reaction is terminated with trichloroacetic acid, and the casein precipitate is removed by centrifugation or filtration, and the supernatant is taken. with NaOH and Na 2 CO 3 After alkalization, add Folin's phenol ...

Embodiment 6-10

[0092] Lycium barbarum extract was dissolved in 5 volumes of deionized water corresponding to Comparative Example 1 to obtain 5 groups of test solutions with different concentrations. When carrying out the inhibition mechanism determination of inhibiting collagenase activity, the mass concentration of Lycium barbarum extract is shown in Table 2 below.

[0093] Determination of the Inhibitory Mechanism of Lycium Barbarum Extract Inhibiting Collagenase Activity

[0094] Add the test solution of 0.25mL comparative example 1 and embodiment 6-10 in 36 test tubes respectively, and add 0.25mL, 0.2mL, 0.15mL, 0.1mL, 0.05mL, 0.025mL collagenase (32U / mL), and Add DPBS buffer solution with a pH of 7.4 to the final volume of 0.5 mL, then add 0.5 mL (1.0%) of the substrate casein solution and mix immediately, keep warm in a water bath at 37°C for 10 min, add 1 mL of trichloroacetic acid solution (6.5%) and mix. After standing for 10 minutes, centrifuge at 10,000 rpm for 5 minutes to obtai...

Embodiment 11-15

[0110] Lycium barbarum extract was dissolved in 5 volumes of deionized water corresponding to Comparative Example 1 to obtain 5 groups of test solutions with different concentrations. When measuring the inhibition mechanism of collagenase activity, the mass concentration of Lycium barbarum extract is shown in Table 3 below.

[0111] Determination of the Inhibition Types of Lycium Barbarum Extracts on Collagenase

[0112] Add 0.3mL, 0.2mL, 0.1mL, 0.05mL of substrate casein solution (1.0%) to 24 test tubes respectively, and add Tris·HCl buffer solution with pH 7.4 to a final volume of 0.5mL, then add 0.25 mL of the test solution of Comparative Example 2 and Examples 11-15, then add collagenase (32U / mL) and mix immediately, after 37 ° C water bath insulation for 10 min, then add 1 mL of trichloroacetic acid solution (6.5%) and mix, statically Centrifuge at 10,000 rpm for 5 minutes after leaving for 10 minutes to obtain the supernatant m of the control group.

[0113] Add 0.3mL,...

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Abstract

The invention discloses a collagenase inhibitor composition, an anti-aging moisture dew and a preparation method of the anti-aging moisture dew. The collagenase inhibitor composition comprises 50-99%of a Chinese wolfberry extract and 1-50% of a ginseng extract, and the mass ratio of the ginseng extract to the Chinese wolfberry extract is preferably 1: 3-15. The anti-aging moisture dew comprises the collagenase inhibitor composition and a penetration enhancer, the addition amount of the collagenase inhibitor composition is 0.01-10%, and the collagenase inhibitor composition comprises 50-99% ofthe Chinese wolfberry extract and 1-50% of the ginseng extract; wherein the addition amount of the penetration enhancer is 0.01%-10%, and the penetration enhancer is bis-diethoxydiethylene glycol cyclohexane 1, 4-dicarboxylate. According to the collagenase inhibitor composition, the Chinese wolfberry extract and the ginseng extract are matched, so that a synergistic effect is achieved, and the activity of collagenase can be well inhibited. The anti-aging moisture dew can reduce degradation of collagen, has an anti-aging effect, also has moisturizing and water locking effects, can prevent moisture loss, and creates a moisturizing barrier for the skin.

Description

technical field [0001] The invention relates to the field of daily chemicals, in particular to a collagenase inhibitor composition, anti-aging lotion and a preparation method thereof. Background technique [0002] Collagen is mainly produced by fibroblasts in the dermis and is the main component of the dermis. It can maintain the structure of the skin and give the skin toughness and elasticity. The content and distribution of collagen determine the youthfulness of the skin. However, the abnormal reduction of collagen content will cause the thinning of the dermis, loose skin, loss of elasticity, and wrinkles, which will affect people's quality of life. With the deepening of research on collagen, researchers have found that collagenase plays an important role in the homeostasis of skin collagen, and its overexpression and abnormal activation are one of the main causes of skin aging. Therefore, inhibiting the activity of collagenase can block the degradation of skin collagen,...

Claims

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Application Information

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IPC IPC(8): A61K8/9789A61K8/37A61Q19/08A61Q19/00A61K36/815A61P17/16A61K8/34A61K8/44A61K8/49A61K8/64A61K8/65A61K8/68A61K8/73A61K8/9711A61K8/9722
CPCA61K8/9789A61K8/37A61Q19/08A61Q19/00A61K36/815A61K36/258A61P17/16A61K8/65A61K8/645A61K8/68A61K8/9711A61K8/9722A61K8/44A61K8/73A61K8/34A61K8/4946A61K2800/782A61K2800/5922A61K2800/85A61K2300/00
Inventor 杨登亮谢佩佩李传茂张楚标曾伟丹张伟杰
Owner GUANGZHOU KENENG COSMETICS RES CO LTD
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