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Rhodosporidium sphaercarpum ZDFY1801 and application thereof

A technology of ZDFY1801 and Rhodosporidium yeast, which is applied in the fields of application, chemicals for biological control, biological water/sewage treatment, etc., can solve the problems such as no reports of marine red yeast, and achieve the effect of fast ammonia nitrogen removal efficiency

Active Publication Date: 2020-09-22
中山大学附属中学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Marine Rhodotorula is mainly used in aquatic bait and production of carotenoids, but there is no report on other uses of Marine Rhodotorula

Method used

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  • Rhodosporidium sphaercarpum ZDFY1801 and application thereof
  • Rhodosporidium sphaercarpum ZDFY1801 and application thereof
  • Rhodosporidium sphaercarpum ZDFY1801 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Screening and isolation of Rhodosporidium sphaeroides ZDFY1801

[0024] Enrichment medium (YPD) composition: yeast extract 10g, peptone 20g, glucose 20g, deionized water 1L; preparation: dissolve each component in deionized water, stir to dissolve, sterilize under high pressure steam at 121°C for 20 minutes, and cool to get ready.

[0025] YPD solid medium is a liquid medium in which 1.5wt% agar powder is added.

[0026] Ammonium Nitrogen Bacteria Isolation Medium (IMAR) Composition: Ammonium Sulfate 0.5g, Glucose 6g, Sucrose 6g, Yeast Extract 0.5g, NaH 2 PO 4 0.5g, 1L of sand-filtered seawater; preparation: dissolve each component in seawater, stir to dissolve, adjust the pH to 7.0, sterilize by high-pressure steam at 121°C for 20 minutes, and cool to obtain.

[0027] Sample source: Mixed samples of prawn breeding water and pond bottom mud in Dianbai District, Maoming City, Guangdong Province.

[0028] The specific implementation steps are as follows: un...

Embodiment 2

[0030] Example 2: Molecular identification of Rhodosporidium globosa ZDFY1801

[0031]Centrifuge the ZDFY1801 strain culture liquid, discard the supernatant, and extract genomic DNA from bacterial cell precipitation. Using genomic DNA as a template, use universal primers EF3 / EF4 (EF3: 5'-TCCTCTAAATGACCAAGTTTG-3'; EF4: 5'-GGAAGGGRTGTATTTATTAG- 3') 18S rDNA was amplified (Smit et al., 1999) and sequenced. The sequence is shown in SEQ ID NO.1 in the sequence listing. The sequence was compared with the Genbank database and analyzed by the phylogenetic tree, and it was identified as Globulin Rhodosporidium sphaercarpum, named: Rhodosporidium sphaercarpum YLY01 (ZDFY1801). The strain was preserved in Guangdong Microbial Culture Collection Center (GDMCC) on June 3, 2019, address: Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou, Guangdong Province, and the preservation number is GDMCC No: 60679.

[0032] Phylogenetic tree of Rhodosporidium globosa ZDFY1801 image 3 . ...

Embodiment 3

[0033] Example 3: Effect of Rhodosporidium globosa ZDFY1801 on the removal of ammonia nitrogen in simulated aquaculture water

[0034] Take aquaculture water samples and add (NH 4 ) 2 SO 4 Adjust the ammonia nitrogen concentration in the water to 10±0.2mg / L, add glucose to a final concentration of 1g / L, filter and sterilize with a 0.2μm filter membrane, and then dispense 150mL into sterilized 250mL Erlenmeyer flasks, three for each of the experimental group and the control group repeat. Take 2 mL of Rhodosporidium globosa ZDFY1801 cultured overnight in YPD medium, wash it twice with sterilized seawater, and then suspend it into 2 mL. Add 500 μL of suspended ZDFY1801 bacterial liquid to the experimental group, and add the same amount of sterilized seawater to the control group. Take 0h, 3h, 6h, 9h, 12h, 24h, 36h, 48h water samples from the clean bench. Use a portable water quality analyzer (Aokedan, Wuxi) to detect the content of ammonia nitrogen and nitrite, and measure th...

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Abstract

The invention discloses a rhodosporidium sphaercarpum ZDFY1801 and application thereof. The preservation number of the strain is GDMCC No: 60679. The strain is identified as rhodosporidium sphaercarpum through a 18S rDNA gene.The strain ZDFY1801 has the capability of efficiently removing ammonia nitrogen in simulated aquaculture water, and also has flocculation and clarification effects on organicsuspended particles in the aquaculture water. The strain ZDFY1801 can also significantly inhibit vibrio of aquaculture water, and the strain ZDFY1801 has high biological safety and also has rich nutrition. The characteristics show that the ZDFY1801 can be used for treating wastewater in aquaculture and can also be used as bait for aquaculture animals or used as a feed additive.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to a strain of Rhodosporidium globosa ZDFY1801 and its application. Background technique [0002] In aquaculture, a large number of water changes are usually used to discharge aquaculture waste to solve the problems of excessive ammonia nitrogen and the proliferation of pathogenic bacteria. With the development of ecological civilization construction and the increasing awareness of environmental protection in all walks of life, it is unsustainable to discharge aquaculture waste into the natural environment with a large number of water changes. The use of aquatic probiotics to improve aquaculture water has gradually been recognized by the majority of aquaculture workers. . However, the currently widely used probiotics for reducing ammonia nitrogen water quality in the market are mainly autotrophic nitrifying microorganisms such as nitrifying bacteria and nitrosifying b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A01N63/32A01P1/00C02F3/34A23K10/18A23K50/80C12R1/645C02F103/20C02F101/16
CPCA01N63/32C02F3/347A23K10/18A23K50/80C02F2103/20C02F2101/16C12R2001/645C12N1/145
Inventor 罗鹏陈如欣云龙徐芃阳莫壘先杨艳君刘勇斌
Owner 中山大学附属中学
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