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A method for culturing tumor tissue slices

A tumor tissue and slice technology, applied in the field of biomedicine, can solve the problems of poor effect and long time, and achieve remarkable results of technological progress

Active Publication Date: 2021-03-30
澎立生物医药技术(上海)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above-mentioned problems in the prior art, the present invention provides a method for culturing tumor slices. The method for cultivating tumor slices needs to solve the problem of long time and predictable drug loss in the method of culturing tissue by organoid method in the prior art. Technical issues with poor efficacy

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  • A method for culturing tumor tissue slices
  • A method for culturing tumor tissue slices
  • A method for culturing tumor tissue slices

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Embodiment 1

[0043] 1. Materials and methods

[0044] 1.1 Collection of patient tumor samples

[0045] Tumor tissues removed from lung cancer patients were collected and immediately placed in precooled CO containing 100ug / mL primary cell antibiotic (primocin). 2 In independent media. Ensure that tumors are kept at 4°C before slicing.

[0046] 1.2 Slice and culture of tumor

[0047] 1) The patient sample is placed in a 10 cm diameter cell culture dish for trimming. The culture dish contains Hank's balanced salt solution, and penicillin and streptomycin are added to the solution. In the solution, the The concentration of the penicillin is 100U / mL, and the concentration of the streptomycin is 100U / mL. The connective tissue and necrotic tissue around the tumor tissue were removed. Cut the tumor tissue into 200-300 μm tumor tissue slices with a vibrating microtome. A single tumor tissue slice was cultured in a 6-well cell culture plate using a 30-mm diameter, 0.4-μm-thick insert cell cult...

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Abstract

The invention provides a method for culturing tumor slices. The method comprises a step of collecting a tumor sample of a patient; a step of slicing the tumor tissue; a step of culturing the tumor tissue; and a step of culturing the slices in a 37 DEG C incubator containing 5% CO2 for 3-7 days, wherein a culture medium is changed every 2-3 days. The culture method disclosed by the invention can beused for carrying out in-vitro primary culture on the primary tissue slices of a tumor. In the culture process, the activity of tumor cells, stromal cells and immune cells infiltrated in the tumor can be ensured. In this way, administration of tumor immunity related drugs can be simulated in vitro, and the medication effect of a patient can be directly detected in vitro.

Description

Technical field: [0001] The invention belongs to the field of biomedicine, and relates to a method for culturing cells, in particular to a method for culturing tumor tissue slices. Background technique: [0002] Two great challenges in current cancer treatment are: (i) tumors are highly heterogeneous and constantly evolving, resulting in drug resistance; (ii) stromal and immune cells are hijacked by tumor cells, rendering them unable to monitor tumorigenesis. Although immunotherapy has successfully cured some patients, we are far from finding effective treatments for most solid tumors. [0003] Solid tumors have a complex tumor microenvironment, and its two main characteristics are: high heterogeneity and complex three-dimensional structure. An ideal laboratory model should have both these two characteristics. Although we have obtained a lot of data in 2D models (in vitro cell culture methods), 2D tumor models based on cell culture cannot reflect the complexity of host-tumo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09
CPCC12N5/0693C12N2500/25C12N2500/30C12N2500/32C12N2500/44C12N2500/84C12N2501/22C12N2501/2302
Inventor 杨楠俞诚成艳张元罗艳段继峰
Owner 澎立生物医药技术(上海)股份有限公司