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Single nucleotide polymorphism (SNP) molecular marker of pinctada fucata martensii growth trait-related pinctada fucata martensii molluscan insulin-related peptide (MIP) gene and application of SNP molecular marker

A technology of pinctada martensii and molecular markers, which is applied in the direction of microbial measurement/inspection, recombinant DNA technology, biochemical equipment and methods, etc., and can solve the problems of affecting the selection effect and reducing the selection accuracy

Active Publication Date: 2020-09-25
GUANGDONG OCEAN UNIVERSITY
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AI Technical Summary

Problems solved by technology

Phenotypic traits are jointly determined by genes and the environment. Environmental interference often affects the selection effect and reduces the accuracy of selection

Method used

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  • Single nucleotide polymorphism (SNP) molecular marker of pinctada fucata martensii growth trait-related pinctada fucata martensii molluscan insulin-related peptide (MIP) gene and application of SNP molecular marker
  • Single nucleotide polymorphism (SNP) molecular marker of pinctada fucata martensii growth trait-related pinctada fucata martensii molluscan insulin-related peptide (MIP) gene and application of SNP molecular marker
  • Single nucleotide polymorphism (SNP) molecular marker of pinctada fucata martensii growth trait-related pinctada fucata martensii molluscan insulin-related peptide (MIP) gene and application of SNP molecular marker

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Experimental program
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Effect test

Embodiment 1

[0031] 1. Genomic DNA extraction

[0032] Use TIANGEN's Marine Animal Tissue Genomic DNA Extraction Kit to extract genomic DNA. The specific steps include:

[0033] (1) Take 0.05 g of adductor muscle and put it into an autoclaved 1.5 mL centrifuge tube containing 200 μL of GA buffer;

[0034] (2) Mince the muscle tissue, add 4 μL of ribonuclease A (100 mg / mL) solution, shake for 15 sec, and place at room temperature for 5 min;

[0035] (3) Add 20 μL of proteinase K (20 mg / mL) solution, vortex to mix, centrifuge briefly, place at 56°C for 3 hours, shake and mix the sample 2-3 times per hour, and mix for 15 sec each time;

[0036] (4) Add 200 μL of buffer solution, mix thoroughly by inversion, place at 70°C for 10 minutes, and briefly centrifuge;

[0037] (5) Add 200 μL of absolute ethanol, mix thoroughly by inversion, and briefly centrifuge;

[0038] (6) Add the solution and flocculent precipitate obtained in the previous step into an adsorption column (the adsorption column...

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Abstract

The invention discloses a single nucleotide polymorphism (SNP) molecular marker of a pinctada fucata martensii growth trait-related pinctada fucata martensii molluscan insulin-related peptide (MIP) gene and application, and belongs to the technical field of molecular marker assisted breeding. The locus corresponds to a gene locus of pinctada fucata martensii molluscan insulin-related peptide (MIP), and an SNP locus of the molecular marker corresponds to a base A or G at the locus 38363939 or a base C or T at the locus 38364205 on the chromosome 3 of pinctada fucata martensii. The SNP marker ofthe invention is significantly related to the growth traits (shell length, shell width, shell height, shell weight and total weight) of pinctada fucata martensii, and is two new molecular markers; bydetermining the SNP locus genotype of to-be-detected pinctada fucata martensii, early breeding is performed on the growth traits of pinctada fucata martensii, so that the breeding cycle is shortened,the genetic progress is improved, and the production cost is reduced; and the SNP molecular marker has obvious economic and social benefits.

Description

technical field [0001] The invention discloses a MIP gene SNP molecular marker related to growth traits of Pinctada martensii and an application thereof, belonging to the technical field of molecular marker-assisted breeding. Background technique [0002] Pinctada fucata martensii is the main mollusc in my country to cultivate seawater nucleated pearls. At a stage in which the output of seawater pearls in my country continues to decline, the cultivation of fine varieties is one of the effective ways to deal with industrial problems. The combination of traditional breeding methods and genotyping technology can improve the accuracy and precision of trait selection, and more importantly, greatly shorten the breeding time, which has become the mainstream direction of current breeding. Cultivating new varieties suitable for local sea area breeding and improving the growth and pearl breeding traits of the cultured population are the primary tasks to solve the industrial predicame...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172Y02A40/81
Inventor 邓岳文郝瑞娟杨创业杜晓东郑哲王庆恒
Owner GUANGDONG OCEAN UNIVERSITY
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