C57 mouse in-vivo virus research step based on experimental detection

Abstract

The invention discloses a C57 mouse in-vivo virus research step based on experimental detection. The research step specifically comprises the following steps: S1, carrying out selection treatment of C57 mouse and H1NI diseased strains: selecting 20 C57 male mice with the body weight of 20-22g, and then taking the H1NI diseased strains to prepare a diluent. The invention relates to the technical field of animal virus research. According to the C57 mouse in-vivo virus research step based on experimental detection, through selection treatment of C57 mouse and H1NI diseased strains, 20 C57 male mice with the body weight of 20-22 g are selected, then the H1NI diseased strains are taken to be prepared into the diluent; tracking information of an H1NI virus is designed: virus RNA is extracted from the diluent prepared from the H1NI diseased strains by adopting a paramagnetic particle method, transcription is performed to synthesize cDNA, fluorescence labeling treatment is carried out on the H1N1 virus, and after carrying out C57 mouse infection by using the virus cell, tracking detection of the content and the position of virus in each mouse body can be realized, so that the effect of researching the content of virus cells detected by an H1N1 experiment on the mice is improved.

Description

technical field [0001] The invention relates to the technical field of animal virus research, in particular to a virus research procedure in C57 mice based on experimental detection. Background technique [0002] H1N1 is an RNA virus belonging to the Orthomyxoviridae family. Its hosts are birds and some mammals. Almost all A-type H1N1 viruses have been isolated. Diseases in wild birds are rare, and some H1N1 viruses cause severe diseases. Most of them occur in poultry and pets, but rarely in humans. However, after the spread and mutation of birds and some mammals, this may lead to epidemics or large-scale spread of human influenza. H1N1 is marked according to the H number (type of hemagglutinin) and an N number (type of neuraminidase), each subtype of avian influenza virus has mutated into various strains with different pathogenicity profiles; some are pathogenic for one species, but not for others Multiple species of disease. [0003] When conducting experimental research...

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Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a research procedure based on experimental detection of viruses in C57 mice, which solves the problem that the current research methods usually cannot track the virus content in mice, resulting in the inability to carry out virus detection in mice. Questions for cell content studies

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