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Novel culture medium preparation method

A culture medium preparation and culture medium technology, applied in biochemical equipment and methods, sterilization methods, biochemical instruments, etc., can solve the problems of poor sealing, influence of medium, and no mixing effect, etc., to achieve good sealing, Good mixing effect, convenient installation and disassembly

Pending Publication Date: 2020-10-02
盐城呈祥园艺育苗有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main purpose of the present invention is to provide a novel medium preparation method, which can effectively solve the problem that under normal circumstances, fungi in the outside air cannot be prevented from entering the medium, the sealing performance is relatively poor, and there is no convenient installation and The disassembly function does not have a good mixing and stirring effect, and the stirring process usually needs to open the medium, so that the external environment will affect the inside of the medium.

Method used

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Examples

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Embodiment

[0030] A novel culture medium preparation method, comprising a base 1 and a cover shell 2, the specific steps are as follows:

[0031] Step 1: Preparation of potato filtrate Preparation: select high-quality potatoes, then peel the potatoes, cut into pieces, boil for soaking and sterilize until the potato filtrate is obtained;

[0032] Step 2: Preparation of moso bamboo powder Preparation: Weigh a number of moso bamboo branches, then clean, sterilize and crush the moso bamboo branches until obtaining moso bamboo powder, and pour it into the base 1;

[0033] Step 3: medium preparation: transfer the potato filtrate and moso bamboo powder prepared in step 2 and step 1 and other components of the nutrient solution to the substrate 1 of the prepared medium;

[0034] Step 4: Addition of nutrient solution: Observe the height of the nutrient solution inside the medium in step 3. When the height of the nutrient solution is lower than the height of the initial nutrient solution, add the ...

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Abstract

The invention discloses a novel culture medium preparation method. The novel culture medium preparation method comprises a substrate and a cover shell, and comprises the following specific steps: step1, preparing potato filtrate, namely selecting high-quality potatoes, and then performing peeling, dicing, boiling, soaking and disinfecting on the potatoes until the potato filtrate is obtained; and2, performing moso bamboo powder preparation: weighing a plurality of moso bamboo branches, performing cleaning, disinfecting and crushing on the moso bamboo branches until the moso bamboo powder isobtained, and pouring the moso bamboo powder into a substrate. According to the preparation method of the novel culture medium, bacteria in external air can be prevented from entering the culture medium, the sealing performance is high, the convenient mounting and dismounting functions are achieved, the good mixing and stirring effect is achieved, the culture medium does not need to be opened in the stirring process, and the culture medium is always in a sealed state; and the influence of the external environment on the interior of the culture medium is avoided.

Description

technical field [0001] The invention relates to the technical field of medium preparation, in particular to a novel medium preparation method. Background technique [0002] Studies have shown that almost all fungi can live in soil, surface litter, oceans, rivers or lakes. At present, when cultivating fungi, common mediums are mostly used for culturing. Most of the existing media only contain potatoes, glucose, and agar, so it is difficult to provide comprehensive and sufficient nutrients, and the quality of the cultured fungi is poor and the yield is low; [0003] There are certain disadvantages in the existing culture medium preparation methods. Generally, it is impossible to prevent fungi in the outside air from entering the culture medium, and the sealing performance is relatively poor, and it does not have convenient installation and disassembly functions, and it is not very good. The mixing and stirring effect, and the stirring process usually needs to open the medium...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/02C12M1/00
CPCC12M27/02C12M23/50C12M37/04
Inventor 陈祥沈羊城陈玉宏
Owner 盐城呈祥园艺育苗有限公司