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One-step method rooting and transplanting technology for tissue culture seedlings of Fraxinus mandshurica

A technology of tissue culture seedlings and Mandshurica mandshurica, applied in the field of forest tree genetics and breeding, can solve the problems of no systematic rooting and transplanting technology, and achieve the effect of saving time, cost and huge economic benefits

Active Publication Date: 2020-10-13
NORTHEAST FORESTRY UNIVERSITY
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Problems solved by technology

The tissue culture of Mandshurica mandshurica mainly obtains adventitious buds through organogenesis (buds, stem segments, hypocotyls), and in order to realize large-scale reproduction, adventitious buds need to be rooted and transplanted. There are only sporadic reports on rooting and transplanting, and no research on systematic rooting and transplanting techniques

Method used

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  • One-step method rooting and transplanting technology for tissue culture seedlings of Fraxinus mandshurica
  • One-step method rooting and transplanting technology for tissue culture seedlings of Fraxinus mandshurica
  • One-step method rooting and transplanting technology for tissue culture seedlings of Fraxinus mandshurica

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Experimental program
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Embodiment Construction

[0011] 1. Effects of four basic media on the growth of Mandshurica mandshurica seedlings

[0012] Adventitious shoots induced by Mandshurica mandshurica callus were inoculated in MS, WPM, B5, IS medium supplemented with 2.0 mg / L 6-BA, 20 g / L sucrose and 7 g / L agar after elongation culture for 28 days. 30 bottles were repeated for each treatment, and one explant was inoculated in each bottle. After 30 days, the plant height, stem diameter, leaf state (leaf number and leaf color) and survival rate were counted.

[0013] Table 1 The effect of the basic medium on the strong seedlings of Mandshurica mandshurica tissue culture seedlings

[0014]

[0015]

[0016] Note: Lowercase letters represent significant differences at the P<0.05 level.

[0017] 2. The influence of the growth state of adventitious branches on the strong seedlings of Mandshurica mandshurica tissue culture seedlings

[0018] Under sterile conditions, semi-lignified single plant seedlings (with callus at t...

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Abstract

The invention provides a one-step method rooting and transplanting technology for tissue culture seedlings of Fraxinus mandshurica. Semi-lignified adventitious individual plants obtained by tissue culture are cultured for 30 d in a strong seedling medium (WPM + 2.0 mg / L 6-BA + 20 g / L sucrose + 7 g / L agar), and then directly planted in a seedling culture bag containing a rooting medium (WPM + 1.4 mg / L IBA+0.7 mg / L NAA, without sucrose and agar) matrix (the ratio of turfy soil to vermiculite is 3:1), then the seedling culture bag is closed with a plastic pull bag, the plastic pull bag is opened7 d after culture under specific light, temperature and humidity conditions, the plastic pull bag is removed after 14 d, after 30 days of constant-temperature and constant-humidity culture, adventitious branches take root and grow out new leaves, and the survival rate is 63.33%. The technology simplifies rooting and transplanting into one step, is one of key technologies for rapid propagation of tissue culture seedlings of the Fraxinus mandshurica, saves the time and cost, and lays a solid foundation for the large-scale production of rapid propagation of the Fraxinus mandshurica.

Description

Technical field: [0001] The invention belongs to the field of genetic breeding of forest trees, the field of plant tissue culture, and high-efficiency propagation technology of Mandshurica mandshurica Background technique: [0002] Fraxinus mandshurica seeds have the characteristics of deep dormancy, the dormancy period is as long as several months, the vernalization treatment time is longer, and the reproduction speed is slow, so it is particularly important to establish an efficient reproduction technology. At the same time, in order to maintain the traits of excellent varieties, shorten the breeding time, and expand the reproduction coefficient, asexual reproduction is also the best choice. The tissue culture of Mandshurica mandshurica mainly obtains adventitious buds through organogenesis (buds, stem segments, hypocotyls), and in order to realize large-scale reproduction, adventitious buds need to be rooted and transplanted. There are only sporadic reports on rooting an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G17/00A01G24/15A01G24/28
CPCA01H4/001A01H4/008A01G17/005A01G24/28A01G24/15
Inventor 詹亚光于磊丁一巍
Owner NORTHEAST FORESTRY UNIVERSITY
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