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CAPS marker for detecting resistance of muskmelon phytophthora blight and application thereof

A marker and melon technology, applied in the field of CAPS markers and their corresponding primers, can solve the problems of no molecular markers, etc.

Active Publication Date: 2020-10-13
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no disease-resistant gene has been mapped or cloned, and no molecular markers closely linked to disease-resistant genes have been reported (currently reported melon molecular markers are basically focused on agronomic traits, powdery mildew resistance, etc.)

Method used

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  • CAPS marker for detecting resistance of muskmelon phytophthora blight and application thereof
  • CAPS marker for detecting resistance of muskmelon phytophthora blight and application thereof
  • CAPS marker for detecting resistance of muskmelon phytophthora blight and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: This embodiment is the SNP sites and markers linked to the blight resistance gene of melon and the method for obtaining them.

[0029] 1. Melon material for testing: the present invention uses the susceptible variety E31 selected and bred by this laboratory as the female parent (P 1 ), with the disease-resistant variety ZQK9 as the male parent (P 2 ), using two parents to prepare F 1 、BC 1 P 1 、BC 1 P 2 and F 2 group.

[0030] Tested strains: The strains used in this experiment were isolated from the diseased melon plants in the Hainan field, and were identified as Phytophthora capsici.

[0031] 2. Analysis methods and results

[0032] (1) Analysis of disease resistance at seedling stage: disease resistance identification adopts root irrigation inoculation method, when the seedling grows to the stage of two leaves and one heart, at the place about 1 cm away from the root of the seedling, pierce a 1 cm deep hole, and inject 1 ml of the concentration...

Embodiment 2

[0059] Example 2: F 2 Genotype verification of population and natural population materials

Embodiment approach

[0061] 1. Experimental materials

[0062] Melon materials tested: resistant parent ZQK9, susceptible parent E31, including 497 individual plants of F 2 groups, and a natural group material consisting of 40 melon materials.

[0063] 2. Experimental method

[0064] The phenotypes of all the experimental materials were identified by the root irrigation inoculation method, and the DNA of all individual plants was extracted by the CTAB method. After PCR amplification using the CAPS marker, it was digested with the restriction endonuclease Fau I and electrophoresed on a polyacrylamide gel. detection ( figure 1 ). Analysis of F using CAPS markers 2 genotype of the population, combined with F 2 The identification results of disease resistance of individual plants in the field were analyzed by using JoinMap4.0 software for linkage genetic analysis.

[0065] 3. Experimental results

[0066] Utilize the CAPS tag for the F 2 After the genotypes of 497 individual plants in the popu...

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Abstract

The invention provides a CAPS marker closely linked with a muskmelon epidemic-disease-resistant gene. The CAPS marker is characterized in that polymorphic sites of the CAPS marker are located at positions of chromosomes 22, 903 and 700 of version 12 of a muskmelon genome 3.5.1 and are G / C polymorphisms; the invention also provides application of the molecular marker in muskmelon breeding, varietyidentification and preparation of a corresponding test kit.

Description

technical field [0001] The application belongs to the technical field of agricultural molecular biology detection. Specifically, the application provides a CAPS marker closely linked with the melon blight resistance gene and its corresponding primers, detection method and application. Background technique [0002] Melon is a Cucurbitaceae Cucumis melo L. crop, which is an important horticultural crop in my country. According to the statistics of the Food and Agriculture Organization of the United Nations, my country's melon cultivation area and output rank first in the world, which has played an important role in increasing farmers' income and promoting the reform of the agricultural supply-side structure. [0003] Epidemic is a devastating soil-borne disease caused by Phytophthora infection. It can be transmitted through rain, soil, and air. High temperature and high humidity environment can promote the occurrence and prevalence of epidemic. Phytophthora can infect the roo...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 王平勇徐志红徐永阳付楠赵光伟贺玉花孔维虎张健
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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