Composition comprising stem cell-derived exosomes for adipogenic differentiation induction, adipose tissue regeneration, skin whitening or wrinkle amelioration

Abstract

The present invention relates to a pharmaceutical composition for adipocyte differentiation induction and / or adipose tissue regeneration comprising, as an active ingredient, an exosome extracted froma stem cell which is differentiating into an adipocyte. The exosome is excellent in expression rate of bioactive factors influencing the differentiation into an adipocyte and has the effect of differentiating a stem cell into an adipocyte. Accordingly, the present invention can be applied to differentiation inducing agents of a stem cell, injections for tissue regeneration, fillers for cosmetic purposes, preparations for tissue engineering, etc. The invention also relates to a cosmetic composition for skin whitening, wrinkle improvement and regeneration, containing an exosome extracted from astem cell as an active ingredient. Since the exosome contains genes, proteins, growth factors etc. associated with the proliferation, differentiation, and regeneration of stem cells; is a purified component that does not include antibiotics, serums or culture solution harmful factors; and is a cell-derived lipid transporter, the exosome can be applied to functional cosmetic compositions having skin whitening, wrinkle improvement and skin regeneration functions and scar improving preparations for cosmetic purposes, etc.

Description

technical field [0001] The present invention relates to a composition for inducing stem cells to differentiate into adipocytes and / or regenerating adipose tissue using stem cell-derived exosomes containing adipocyte differentiation-inducing substances. In addition, the present invention relates to a cosmetic composition comprising stem cell-derived exosomes for skin whitening, wrinkle improvement or skin regeneration. Background technique [0002] As a therapeutic method for regenerating adipose tissue, there is a method using a therapeutic agent including stem cells cultured using a matrix having adipose tissue three-dimensionally cultured in a hydrogel. Growth factors and extracellular matrix can be secreted by stem cells after three-dimensional culture of the tissue in a hydrogel. However, in order for the therapeutic agent to be used as an injectable dosage form, it is inconvenient to remove the hydrogel in thin film form from the culture vessel and to treat the tissue ...

AI Technical Summary

Problems solved by technology

Thus, in the currently used stem cell-derived exosome isolation method, it is difficult to completely purify exosomes due to the intervention of proteins in the medium or serum in the step of isolating the secretome including exosomes.

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