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Preparation method of high-strength DNA hydrogel with macroporous structure

A hydrogel, high-strength technology, applied in the field of DNA hydrogels, which can solve the problems of inability to respond and improve mechanical properties

Active Publication Date: 2020-10-20
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the above methods can effectively improve the response speed or mechanical properties of DNA hydrogels, they often need to add additional additives, and cannot improve the response speed and mechanical properties at the same time.

Method used

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  • Preparation method of high-strength DNA hydrogel with macroporous structure
  • Preparation method of high-strength DNA hydrogel with macroporous structure
  • Preparation method of high-strength DNA hydrogel with macroporous structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method of DNA hydrogel with macroporous structure is as follows:

[0033] 10mM / L Tris-HCl at pH 8.0, 50mM / L MgCl 2 Adding a final concentration of 2% acrylamide solution in the buffer solution, a final concentration of 1mM / L DNA (1) solution, the reaction solution with N 2 Carry out deoxygenation for 5 minutes. After deoxygenation is completed, add APS with a final concentration of 0.4% and TEMED with a final concentration of 0.2%. After that, quickly place the PCR tube containing the reaction solution at -20°C to make the polymerization reaction at low temperature. next. After the polymerization reaction is completed, the cryogel is thawed at 4°C to melt the ice crystals, and a DNA hydrogel with a macroporous structure is obtained. The obtained DNA hydrogel is soaked in a buffer solution to remove unreacted monomers and initiators. agent.

Embodiment 2

[0035] A preparation method of DNA hydrogel with macroporous structure is as follows:

[0036] 10mM / L Tris-HCl at pH 8.0, 50mM / L MgCl 2 Adding a final concentration of 2% acrylamide solution in the buffer solution, a final concentration of 1mM / L DNA (1) solution, the reaction solution with N 2 Carry out deoxygenation for 5 minutes. After deoxygenation is completed, add APS with a final concentration of 0.4% and TEMED with a final concentration of 0.2%. After that, quickly place the PCR tube containing the reaction solution under the condition of -40°C to make the polymerization reaction at low temperature. next. After the polymerization reaction is completed, the cryogel is thawed at 4°C to melt the ice crystals to obtain a DNA hydrogel with a macroporous structure, and the obtained DNA hydrogel is soaked in a buffer solution to remove unreacted monomers and Initiator.

Embodiment 3

[0038] Preparation of traditional DNA hydrogels

[0039] 10mM / L Tris-HCl at pH 8.0, 50mM / L MgCl 2 Adding a final concentration of 2% acrylamide solution in the buffer solution, a final concentration of 1mM / L DNA (1) solution, the reaction solution with N 2 Carry out deoxygenation for 5 minutes, add APS with a final concentration of 0.4% and TEMED with a final concentration of 0.2% after the deoxygenation is completed, and then use N 2 Deoxygenation was carried out for 3 minutes, and then the PCR tube containing the reaction solution was placed at 4° C. to allow the polymerization reaction to proceed. After the polymerization reaction is completed, a traditional DNA hydrogel is obtained, and the obtained DNA hydrogel is soaked with a buffer solution to remove unreacted monomers and initiators.

[0040] The DNA sequence in the DNA (1) solution used in Examples 1-3 is: 5' / Acry / -AAACCTGAATTCAGG, the DNA chain contains a palindromic sequence, and self-complementarity can occur be...

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Abstract

The invention provides a preparation method of high-strength responsive DNA hydrogel with a macroporous structure. According to the preparation method, a polymer monomer and a DNA chain with a modification group at a 5' end are concentrated into a micro-liquid phase around an ice crystal under a freezing condition, and polymerization is conducted under the action of an initiator; i.e., DNA is grafted to a polymer chain through a polymerization reaction between DNA modification groups and a polymer monomer; due to interaction between DNA chains, two or more DNA chains can be connected together,so the DNA serves as a cross-linking unit to connect polymer chains into a polymer network, and the DNA hydrogel is formed; the ice crystal functions as a pore-foaming agent, and after the reaction is finished, temperature is raised, so the ice crystals are melted, and the interconnected macroporous structure is left; and in the process, a freezing polymerization method is introduced into preparation of the DNA hydrogel, the obtained DNA hydrogel is good in mechanical property and high in response speed, and the application field of the DNA hydrogel is widened.

Description

technical field [0001] The invention belongs to the technical field of DNA hydrogel, and in particular relates to a preparation method of a high-strength DNA hydrogel with a macroporous structure. Background technique [0002] Hydrogel is a kind of solid material formed by combining hydrophilic materials with three-dimensional cross-linked network structure and water molecules. It is widely used in drug delivery, tissue engineering, biosensing and other fields. [0003] Among them, DNA hydrogels with three-dimensional network structure can be prepared by using DNA as the cross-linking unit. Due to the codability of DNA sequences, the design of DNA sequences can make it respond to external stimuli, such as ionic strength, pH and temperature, thereby changing the properties of DNA hydrogels and making hydrogels develop in the direction of "smart". , so that its application in new biomedicine and biomaterials has attracted more attention. According to the composition of DNA ...

Claims

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Application Information

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IPC IPC(8): C08J9/28C08J3/075C08F289/00C08F220/56C08F220/54C08L99/00C08L33/26
CPCC08J9/28C08J3/075C08F289/00C08J2399/00C08J2433/26C08J2333/26C08J2499/00C08J2201/0484C08F220/56C08F220/54
Inventor 郭玮炜杜晓雪
Owner NANKAI UNIV
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