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Arthrobacter sp. WN18 and application thereof

A technology of Arthrobacter, microbial strains, applied in bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc.

Active Publication Date: 2020-10-20
JINAN UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the degradation of dioxane by Arthrobacter sp.

Method used

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  • Arthrobacter sp. WN18 and application thereof
  • Arthrobacter sp. WN18 and application thereof
  • Arthrobacter sp. WN18 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Isolation of strains

[0031] a, the preparation of ammonium salt inorganic salt medium (AMS)

[0032] 1L ammonium salt medium includes the following components: 100ml of 10-fold concentrated salt solution, 1.0mL of stock solution A, 20mL of 1.0M phosphate buffer and 1.0mL of trace elements, with a pH of about 7.08.

[0033] l0 times concentrated salt solution: 6.6g / L (NH 4 ) 2 SO 4 , 10.0g / L MgSO 4 ·7H 2 O, 0.15g / L CaCl 2 ·6H 2 O.

[0034] Stock solution A: 5.0g / L Fe-EDTA, 2.0g / L Na 2 MO 4 4H 2 O.

[0035] 1.0M phosphate buffer: 2.26g / L KH 2 PO 4 , 0.94g / L Na 2 HPO 4 .

[0036] Trace elements: 0.25g / L EDTA, 0.5g / L FeSO 4 ·7H 2 O, 0.4g / L ZnSO 4 ·7H 2 O, 0.01g / LNiCl 2 ·6H 2 O, 0.015g / L H 3 BO 3 , 0.05g / L CoCl 2 ·6H 2 O, 0.005g / L CuCl 2 2H 2 O, 0.02g / LMnSO 4 ·H 2 O. The components were autoclaved at 121°C for 15 minutes.

[0037] b. Phosphate buffered saline (PBS) (per liter): 0.24g KH 2 PO 4 , 1.44 g Na 2 HPO 4 , 8.0g NaCl, 0.2g KCl...

Embodiment 2

[0047] Strain WN18 was cultured in AMS medium containing 200 mg / L tetrahydrofuran, and incubated at 30° C. in a rotary shaker at 160 rpm for 48 hours. Cells were harvested in exponential phase by centrifugation at 12,000g for 5 min at 4°C, and the cell pellet was washed 3 times with sterile PBS buffer, then resuspended in fresh AMS for further studies. In each 100ml glass vial, add 20ml of AMS, and add different concentrations of tetrahydrofuran and / or dioxane, then inoculate the resuspended cells with the initial concentration of 0.01Abs (OD600nm), and seal with a butyl rubber stopper, and The cultivation was performed at 160 rpm and 30°C in a rotary shaker.

[0048] a. Add 100mg / L of tetrahydrofuran and dioxane respectively, and their co-metabolism degradation results are as follows image 3 As shown, when the two substrates were added to 100 mg / L at the same time, strain WN18 preferentially removed THF, and then began to degrade dioxane after the concentration of THF dropp...

Embodiment 3

[0052] In order to evaluate the environmental adaptability of WN18 bacteria to co-metabolize and degrade dioxane under different pH and temperature conditions with tetrahydrofuran as the induction substrate. The initial pH of the AMS was adjusted to 3.0, 5.0, 7.0, 9.0 and 11.0. The degradation experiments were carried out under the conditions of AMS (pH 7.1) under natural conditions at temperatures of 4°C, 15°C, 25°C, 35°C, and 45°C.

[0053] The experimental results of the WN18 strain of the present invention under different conditions are as follows: Figure 6 and 7 shown. The results show that the strain can be used at pH (5.0-11.0) ( Figure 6 ) and temperature (4-42°C) ( Figure 7 ) co-metabolic degradation of dioxane. Among them, the optimum pH and temperature are 7.0 and 35°C, respectively. The pH value of 3.0 completely inhibited the removal of dioxane, and it still had strong dioxane degradation ability at 11.0. When the temperature increased from 15°C to 35°C,...

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PUM

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Abstract

The invention provides an Arthrobacter sp. WN18. The Arthrobacter sp. WN18 is preserved in the China General Microbiological Culture Collection Center on June 24, 2020 at No.3, Yard No.1, Beichen WestRoad, Chaoyang District, Beijing, and has a preservation number of CGMCC NO.20135. The strain has the capability of efficiently degrading dioxane and the relatively high environmental adaptability under the condition that tetrahydrofuran is used as a primary substrate, and the reaction activity of the strain can be kept in high-concentration dioxane and tetrahydrofuran. The strain provides an excellent strain resource for biological treatment of the dioxane polluted environment.

Description

technical field [0001] The invention relates to a strain of Arthrobacter WN18 and its application. Background technique [0002] Dioxane, a new type of organic pollutant, is a cyclic ether that widely exists in surface water, groundwater, and soil. It has strong water solubility, stable structure, is not easy to be degraded, and has potential carcinogenicity. It is gradually attracting extensive attention in the field of site restoration. . Dioxane is widely used in pharmaceuticals, cosmetics, paints, deodorants, and as a stabilizer for chlorinated solvents, such as 1,1,1-trichloroethane. In addition, in industrial processes, especially in the process of polyurethane synthetic leather and amino acid synthetic leather, dioxane is often produced as a by-product. As a result, the World Health Organization (WHO) included dioxane in its guidelines for drinking water production in 2003, and in September 2009 it was added to the final third Candidate List of Drinking Water Contam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/06C02F101/34
CPCC02F3/34C02F2101/34C12R2001/06C12N1/205
Inventor 刘娜王棚曲丹催庭晨
Owner JINAN UNIVERSITY
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