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Composite material for bioseparations

A technology of composite materials and raw materials, applied in the direction of ion exchange, alkali metal compounds, ion exchange regeneration, etc., can solve the problems that do not mention the granular material base material

Active Publication Date: 2020-10-23
キラルテクノロジーズヨーロッパエスアーエス
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No mention of particulate material substrates in this reference

Method used

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  • Composite material for bioseparations
  • Composite material for bioseparations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0172] 15 ml of an aqueous solution of polymer A2 (11% polymer A2 in solution) was mixed with a solution (704 μl) of 1,6-hexanediol diglycidyl ether (HDGE) to achieve 7-9% crosslinker. The crosslinker ratio is calculated considering the number of reactive groups relative to the vinylamine units present in the polymer solution used for the reaction. After mixing, the pH was adjusted to 11 with 0.5 NaOH.

[0173] 10 g of dry powdered porous carrier B1 were deposited in a flat-bottomed stainless steel dish with a diameter of 8 cm. The porous support B1 was impregnated with 39.5 g of a polymer-crosslinker solution which was added dropwise and distributed evenly on the porous support and mixed using a spatula. The resulting paste was shaken on a rotary shaker at 600 rpm for 1 minute to obtain a homogeneous mass with a smooth surface. After covering the pan with a stainless steel lid, the paste was heated in an oven at 60° C. without further mixing or moving for 48 hours to yield ...

Embodiment 2 to 4 and comparative example 1 and 2

[0176] Examples 2 to 4 and Comparative Examples 1 and 2 were prepared in the same manner as Example 1 except using the starting materials listed in Table 4.

[0177] Table 4:

[0178] polymer porous carrier Crosslinker ratio crosslinking agent Example 1 A2 B1 7-9％ HDGE Example 2 A3 B1 7-9％ HDGE Example 3 A2 B2 7-9％ BDGE Example 4 A2 B3 7-9％ BDGE Comparative example 1 A1 B1 7-9％ HDGE Comparative example 2 A1 B1 7-9％ BDGE

[0179] Determination of consumption performance and hlgG recovery rate of composite material in the embodiment

[0180] To measure the decontamination capabilities of composite materials, the degree of depletion (separation) of impurities or undesired compounds from target substances is determined. For this purpose, selectivity assays were used to determine the concentrations of the individual components in the feed. After the purification step, the concentration measurement...

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Abstract

The present invention relates to composite materials useful for purifying proteins obtained from biological feedstocks. The composite materials of the invention comprise a porous support having an average pore size of 5 to 500 nm, said porous support being filled with a polymer which is cross-linked, wherein the polymer is selected from polyvinylamines or polyallylamines having a weight average molecular weight (Mw) of 2,000 to 500,000 Da and a hydrolysis degree of the formamide groups of at least 66%, with the proviso that a polyvinylamine having a weight average molecular weight (Mw) of 27,200 Da and a hydrolysis degree of 70% and a polyvinylamine having a weight average molecular weight (Mw) of 50,000 Da and a hydrolysis degree of 95% are excluded.

Description

technical field [0001] The present invention relates to composite materials for the purification of proteins obtained from biological sources. Background technique [0002] During the past decades, the relevance of proteins as biopharmaceuticals has continued to increase in many therapeutic and diagnostic applications. One area of ​​particular interest is the use of recombinant monoclonal antibodies (mAbs). The number of approved therapeutic mAbs and their fragments for the treatment of inflammatory diseases, diabetes, various cancers, and blood disorders has increased year by year. [0003] Due to the pharmacokinetic properties of mAbs, in many cases an initial single dose of approximately 0.1-1 g per patient is required, followed by similar doses given weekly or monthly. Therefore, large quantities of therapeutic mAbs are required, and thus therapeutic mAbs must be manufactured on an industrial scale. mAbs are produced in biological sources such as fermentation broths (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01D15/08B01J20/285B01J20/32C07K1/16B01J20/28
CPCC07K1/16B01D15/08B01J20/267B01J20/28078B01J20/285B01J20/3204B01J20/3282B01J20/28083B01J20/28085C07K16/00
Inventor 张彤P·佛朗哥森下康人K·戈特沙尔
Owner キラルテクノロジーズヨーロッパエスアーエス