Compound and composition thereof for manufacture of medicine for enhancing mitochondrial activity of nerve cells
A technology of nerve cells and compounds, applied in the field of plant extracts, can solve the problem of lack of effective treatment of such diseases
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Embodiment 1
[0037] Purification of compounds that enhance the mitochondrial activity of nerve cells from the rhizome of Gaoliangyue peach
[0038] In order to obtain the compound that improves the activity of the mitochondria of nerve cells, a water extract of the rhizome of Galangal officinalis (also known as Galangal officinalis) was firstly prepared. In short, the rhizome of Gao Liangyue peach is washed, dried, cut into pieces, and then crushed into about 1 to 2 cm by a grinder. Thereafter, the water extract of the rhizome of Galanga moon peach can be obtained by extracting the pulverized rhizome of Galanga moon peach with water as a solvent. The weight ratio of the solvent to the pulverized product ranges from 10:1 to 5:1. The extraction temperature is between 70°C and 100°C, preferably between 80°C and 90°C. The extraction time in this embodiment is 1 to 2 hours.
[0039] In order to remove residual solids, after the water extract of the rhizome of Galanga galanga obtained through...
Embodiment 2
[0050] Compounds 1-3 promote mitochondrial activity of nerve cells
[0051] In this example, the mitochondrial activity assay was used to test the effect of compound 1, 2, 3 or 4 (concentration: 10 μg / mL) on improving the mitochondrial activity of nerve cells, and the results were as follows image 3 Shown; * and ** in the figure represent p image 3 , compared with the control group N2a cells treated only with cell culture medium, the treatment of compounds 1, 2, and 3 all significantly increased the mitochondrial activity in N2a cells. Specifically, compound 1, 2, or 3 increased mitochondrial activity by about 100%, 29.9%, and 39.9%, respectively. However, this enhancing effect of compound 4 was not observed.
Embodiment 3
[0053] Compounds 1-3 inhibit the production of reactive oxygen species in nerve cells
[0054]In this example, the reactive oxygen species generation test was used to test the inhibitory effect of compound 1, 2, 3, or 4 (with a concentration of 10 μg / mL) on the generation of reactive oxygen species in nerve cells stimulated by hydrogen peroxide. The results are as follows Figure 4 Shown; * and ** in the figure represent p Figure 4 , compared to N2a cells treated with 1 mM hydrogen peroxide, simultaneous administration of compound 1, 2, or 3 significantly reduced the accumulation of reactive oxygen species in N2a cells. Specifically, compounds 1, 2, 3, and 4 decreased the relative content of active oxygen species by about 60.5%, 77.8%, 34.2%, and 28.5%, respectively.
[0055] In summary, the present invention reveals that the administration of trans-p-coumaryl alcohol, trans-p-acetoxycinnamyl alcohol, and piperonol-β-D-glucoside can significantly increase the activity of mitoc...
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