A recovery method of human adipose-derived mesenchymal stem cells after cryopreservation
A high-quality stem cell and ultra-low temperature technology, applied in the field of stem cells, can solve the problems of increased risk and increased cell contamination, and achieve the effects of ensuring safety, good clinical application prospects, and increasing the number and survival rate
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preparation example 1～4
[0037] A cell culture solution A, which comprises 95-97 parts of basal medium and 3-5 parts of serum substitute.
[0038] The specific parameters of Preparation Examples 1-4 are shown in Table 1 below.
[0039] Table 1. Proportions of basal medium and serum substitute in cell culture solution A of Preparation Examples 1-4.
[0040]
[0041] The serum substitutes in Preparation Examples 1 and 2 are Helios company GMP grade serum substitutes,
[0042] The serum substitutes in Preparation Examples 3 and 4 are GMP grade serum substitutes from PALL Company.
preparation example 5～8
[0043] Preparation examples 5-8, a cell culture solution A, the components of which are 95-97 parts of basal medium, 3-5 parts of serum substitute, and 0.2-0.32 parts of rehmannia oligosaccharide.
[0044] The specific parameters of Preparation Examples 5-8 are shown in Table 2 below.
[0045] Table 2. Proportions of basal medium, serum substitute and rehmannia oligosaccharide in cell culture solution A of Preparation Examples 5-8.
[0046] Preparation Example 5 Preparation Example 6 Preparation Example 7 Preparation example 8 Basal culture medium / part 95 95 97 96 Serum replacement / part 3 5 3 4 Rehmannia oligosaccharide / part 0.2 0.26 0.3 0.32
[0047] The serum substitutes in Preparation Examples 1 and 2 are Helios company GMP grade serum substitutes,
[0048] The serum substitutes in Preparation Examples 3 and 4 are GMP grade serum substitutes from PALL Company.
preparation example 9～12
[0050] A culture medium is composed of 100 parts of basal culture medium and 0.24-0.3 parts of paeoniflorin.
[0051] The specific parameters of Preparation Examples 9-12 are shown in Table 3 below.
[0052] Table 3. Proportions of basal medium and paeoniflorin in the culture medium of Preparation Examples 9-12.
[0053] Preparation Example 9 Preparation Example 10 Preparation Example 11 Preparation Example 12 Basal culture medium / part 100 100 100 100 Paeoniflorin / part 0.24 0.26 0.28 0.3
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